Human PRKAA1 ELISA Kit

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  • Alternative name

    Human AMPK subunit alpha-1 ELISA Kit;Human acetyl-CoA carboxylase kinase ELISA Kit;Human ACACA kinase ELISA Kit;Human hydroxymethylglutaryl-CoA reductase kinase ELISA Kit;Human HMGCR kinase ELISA Kit;Human tau-protein kinase PRKAA1 ELISA Kit;Human AMPK1 ELISA Kit;Human AMPK ELISA Kit;Human AMPKa1 ELISA Kit;Human protein kinase AMP-activated catalytic subunit alpha 1 ELISA Kit;Human 5'-AMP-activated protein kinase catalytic subunit alpha-1 ELISA Kit;Human 5'-AMP-activated protein kinase, catalytic alpha-1 chain ELISA Kit;Human AMP -activate kinase alpha 1 subunit ELISA Kit;Human AMP-activated protein kinase, catalytic, alpha-1 ELISA Kit;Human AMPK alpha 1 ELISA Kit;Human protein kinase, AMP-activated, alpha 1 catalytic subunit ELISA Kit;

  • Catalog
    E031651
  • species
    Human
  • GenePRKAA1
  • Standard CurveHuman PRKAA1 ELISA Kit
  • Other Species Mouse Prkaa1 ELISA KitPorcine PRKAA1 ELISA KitRat Prkaa1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Kinase, AMP Activated Alpha 1 (PRKAa1). No significant cross-reactivity or interference between Protein Kinase, AMP Activated Alpha 1 (PRKAa1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.067ng/mL.
  • Intended UseHuman PRKAA1 ELISA Kit allows for the in vitro quantitative determination of PRKAA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, AMP Activated Alpha 1 (PRKAa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, AMP Activated Alpha 1 (PRKAa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, AMP Activated Alpha 1 (PRKAa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase, AMP Activated Alpha 1 (PRKAa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human 5'-AMP-activated protein kinase catalytic subunit alpha-1 Protein information
  • Uniprot ID AAPK1_HUMAN
  • Uniprot AC Q13131; A8MTQ6; B2R7E1; O00286; Q5D0E1; Q86VS1; Q9UNQ4;
  • UniGene Hs.43322;
  • GeneID 5562
  • KEGG hsa:5562;
  • Human 5'-AMP-activated protein kinase catalytic subunit alpha-1 Protein SEQUENCE
  • SEQUENCE 559 AA; 64009 MW; ABAE71FBF912947A CRC64;

    MRRLSSWRKM ATAEKQKHDG RVKIGHYILG DTLGVGTFGK VKVGKHELTG

    HKVAVKILNR QKIRSLDVVG KIRREIQNLK LFRHPHIIKL YQVISTPSDI

    FMVMEYVSGG ELFDYICKNG RLDEKESRRL FQQILSGVDY CHRHMVVHRD

    LKPENVLLDA HMNAKIADFG LSNMMSDGEF LRTSCGSPNY AAPEVISGRL

    YAGPEVDIWS SGVILYALLC GTLPFDDDHV PTLFKKICDG IFYTPQYLNP

    SVISLLKHML QVDPMKRATI KDIREHEWFK QDLPKYLFPE DPSYSSTMID

    DEALKEVCEK FECSEEEVLS CLYNRNHQDP LAVAYHLIID NRRIMNEAKD

    FYLATSPPDS FLDDHHLTRP HPERVPFLVA ETPRARHTLD ELNPQKSKHQ

    GVRKAKWHLG IRSQSRPNDI MAEVCRAIKQ LDYEWKVVNP YYLRVRRKNP

    VTSTYSKMSL QLYQVDSRTY LLDFRSIDDE ITEAKSGTAT PQRSGSVSNY

    RSCQRSDSDA EAQGKSSEVS LTSSVTSLDS SPVDLTPRPG SHTIEFFEMC

    ANLIKILAQ

  • UCSC uc003jmb.4; human. [Q13131-1];


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