Human PRKCG ELISA Kit

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  • Alternative name

    Human PKC-gamma ELISA Kit;Human PKCG ELISA Kit;Human PKCC ELISA Kit;Human SCA14 ELISA Kit;Human protein kinase C gamma ELISA Kit;Human protein kinase C gamma type ELISA Kit;

  • Catalog
    E031621
  • species
    Human
  • GenePRKCG
  • Other Species Mouse Prkcg ELISA KitBovine PRKCG ELISA KitMonkey PRKCG ELISA KitRabbit PRKCG ELISA KitRat Prkcg ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human PRKCG. No significant cross-reactivity or interference between human PRKCG and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human PRKCG is typically less than 5.86 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman PRKCG ELISA Kit allows for the in vitro quantitative determination of PRKCG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRKCG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRKCG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKCG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKCG bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Protein kinase C gamma type Protein information
  • Uniprot ID KPCG_HUMAN
  • Uniprot AC P05129; B7Z8Q0;
  • UniGene Hs.631564;
  • GeneID 5582
  • KEGG hsa:5582;
  • Human Protein kinase C gamma type Protein SEQUENCE
  • SEQUENCE 697 AA; 78448 MW; 3F911B5BEF713C41 CRC64;

    MAGLGPGVGD SEGGPRPLFC RKGALRQKVV HEVKSHKFTA RFFKQPTFCS

    HCTDFIWGIG KQGLQCQVCS FVVHRRCHEF VTFECPGAGK GPQTDDPRNK

    HKFRLHSYSS PTFCDHCGSL LYGLVHQGMK CSCCEMNVHR RCVRSVPSLC

    GVDHTERRGR LQLEIRAPTA DEIHVTVGEA RNLIPMDPNG LSDPYVKLKL

    IPDPRNLTKQ KTRTVKATLN PVWNETFVFN LKPGDVERRL SVEVWDWDRT

    SRNDFMGAMS FGVSELLKAP VDGWYKLLNQ EEGEYYNVPV ADADNCSLLQ

    KFEACNYPLE LYERVRMGPS SSPIPSPSPS PTDPKRCFFG ASPGRLHISD

    FSFLMVLGKG SFGKVMLAER RGSDELYAIK ILKKDVIVQD DDVDCTLVEK

    RVLALGGRGP GGRPHFLTQL HSTFQTPDRL YFVMEYVTGG DLMYHIQQLG

    KFKEPHAAFY AAEIAIGLFF LHNQGIIYRD LKLDNVMLDA EGHIKITDFG

    MCKENVFPGT TTRTFCGTPD YIAPEIIAYQ PYGKSVDWWS FGVLLYEMLA

    GQPPFDGEDE EELFQAIMEQ TVTYPKSLSR EAVAICKGFL TKHPGKRLGS

    GPDGEPTIRA HGFFRWIDWE RLERLEIPPP FRPRPCGRSG ENFDKFFTRA

    APALTPPDRL VLASIDQADF QGFTYVNPDF VHPDARSPTS PVPVPVM

  • UCSC uc002qcq.2; human. [P05129-1];


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