Human PRKCE ELISA Kit

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  • Alternative name

    Human nPKC-epsilon ELISA Kit;Human PKCE ELISA Kit;Human protein kinase C epsilon ELISA Kit;Human protein kinase C epsilon type ELISA Kit;

  • Catalog
    E031601
  • species
    Human
  • GenePRKCE
  • Standard CurveHuman PRKCE ELISA Kit
  • Other Species Mouse Prkce ELISA KitRabbit PRKCE ELISA KitRat Prkce ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PRKCE . No significant cross-reactivity or interference between PRKCE and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 0.938ng/ml
  • Intended UseHuman PRKCE ELISA Kit allows for the in vitro quantitative determination of PRKCE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- PRKCE antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- PRKCE antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PRKCE amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PRKCE can be calculated.
  • Human Protein kinase C epsilon type Protein information
  • Uniprot ID KPCE_HUMAN
  • Uniprot AC Q02156; B0LPH7; Q32MQ3; Q53SL4; Q53SM5; Q9UE81;
  • UniGene Hs.580351; Hs.677120;
  • GeneID 5581
  • KEGG hsa:5581;
  • Human Protein kinase C epsilon type Protein SEQUENCE
  • SEQUENCE 737 AA; 83674 MW; 85032D0A091A1F7F CRC64;

    MVVFNGLLKI KICEAVSLKP TAWSLRHAVG PRPQTFLLDP YIALNVDDSR

    IGQTATKQKT NSPAWHDEFV TDVCNGRKIE LAVFHDAPIG YDDFVANCTI

    QFEELLQNGS RHFEDWIDLE PEGRVYVIID LSGSSGEAPK DNEERVFRER

    MRPRKRQGAV RRRVHQVNGH KFMATYLRQP TYCSHCRDFI WGVIGKQGYQ

    CQVCTCVVHK RCHELIITKC AGLKKQETPD QVGSQRFSVN MPHKFGIHNY

    KVPTFCDHCG SLLWGLLRQG LQCKVCKMNV HRRCETNVAP NCGVDARGIA

    KVLADLGVTP DKITNSGQRR KKLIAGAESP QPASGSSPSE EDRSKSAPTS

    PCDQEIKELE NNIRKALSFD NRGEEHRAAS SPDGQLMSPG ENGEVRQGQA

    KRLGLDEFNF IKVLGKGSFG KVMLAELKGK DEVYAVKVLK KDVILQDDDV

    DCTMTEKRIL ALARKHPYLT QLYCCFQTKD RLFFVMEYVN GGDLMFQIQR

    SRKFDEPRSR FYAAEVTSAL MFLHQHGVIY RDLKLDNILL DAEGHCKLAD

    FGMCKEGILN GVTTTTFCGT PDYIAPEILQ ELEYGPSVDW WALGVLMYEM

    MAGQPPFEAD NEDDLFESIL HDDVLYPVWL SKEAVSILKA FMTKNPHKRL

    GCVASQNGED AIKQHPFFKE IDWVLLEQKK IKPPFKPRIK TKRDVNNFDQ

    DFTREEPVLT LVDEAIVKQI NQEEFKGFSY FGEDLMP

  • UCSC uc002rut.4; human.;


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