Human PKC ELISA Kit

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  • Catalog
    E031580
  • species
    Human
  • GenePKC
  • Standard CurveHuman PKC ELISA Kit
  • Other Species Human P-PKC ELISA KitHuman P PKC ELISA KitMouse P-PKC ELISA KitMouse PKC ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PKC. No significant cross-reactivity or interference between PKC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PKC and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PKC ELISA Kit allows for the in vitro quantitative determination of PKC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This PKC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PKC. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||PKC ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PKC. Standards or samples are then added to the microtiter plate wells and PKC if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PKC present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PKC are added to each well to "sandwich" the PKC immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PKC and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKC concentration in each sample is interpolated from this standard curve.



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