Human PDIA6 ELISA Kit

Alternative name
Human endoplasmic reticulum protein 5 ELISA Kit;Human ER protein 5 ELISA Kit;Human ERP5 ELISA Kit;Human protein disulfide isomerase P5 ELISA Kit;Human thioredoxin domain-containing protein 7 ELISA Kit;Human P5 ELISA Kit;Human TXNDC7 ELISA Kit;Human protein disulfide isomerase family A member 6 ELISA Kit;Human protein disulfide-isomerase A6 ELISA Kit;Human protein disulfide isomerase-associated 6 ELISA Kit;Human protein disulfide isomerase-related protein ELISA Kit;Human thioredoxin domain containing 7 (protein disulfide isomerase) ELISA Kit;
Catalog
E031530
species
Human
GenePDIA6
Standard Curve
Other Species Mouse Pdia6 ELISA Kit Rat Pdia6 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase A6 (PDIA6). No significant cross-reactivity or interference between Protein Disulfide Isomerase A6 (PDIA6) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.049ng/mL.
Intended UseHuman PDIA6 ELISA Kit allows for the in vitro quantitative determination of PDIA6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A6 (PDIA6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A6 (PDIA6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase A6 (PDIA6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Disulfide Isomerase A6 (PDIA6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Protein disulfide-isomerase A6 Protein information
Uniprot ID PDIA6_HUMAN
Uniprot AC Q99778;
UniGene Hs.212102; Hs.580464;
GeneID 10130
KEGG hsa:10130;
Human Protein disulfide-isomerase A6 Protein SEQUENCE
SEQUENCE 440 AA; 48121 MW; 06895409F0265D7C CRC64;
MALLVLGLVS CTFFLAVNGL YSSSDDVIEL TPSNFNREVI QSDSLWLVEF
YAPWCGHCQR LTPEWKKAAT ALKDVVKVGA VDADKHHSLG GQYGVQGFPT
IKIFGSNKNR PEDYQGGRTG EAIVDAALSA LRQLVKDRLG GRSGGYSSGK
QGRSDSSSKK DVIELTDDSF DKNVLDSEDV WMVEFYAPWC GHCKNLEPEW
AAAASEVKEQ TKGKVKLAAV DATVNQVLAS RYGIRGFPTI KIFQKGESPV
DYDGGRTRSD IVSRALDLFS DNAPPPELLE IINEDIAKRT CEEHQLCVVA
VLPHILDTGA AGRNSYLEVL LKLADKYKKK MWGWLWTEAG AQSELETALG
IGGFGYPAMA AINARKMKFA LLKGSFSEQG INEFLRELSF GRGSTAPVGG
GAFPTIVERE PWDGRDGELP VEDDIDLSDV ELDDLGKDEL
UCSC uc002rau.5; human. [Q15084-1];

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Human PDIA6 ELISA Kit