Human PDIA5 ELISA Kit

Alternative name
Human protein disulfide isomerase-related protein ELISA Kit;Human PDIR ELISA Kit;Human protein disulfide isomerase family A member 5 ELISA Kit;Human protein disulfide-isomerase A5 ELISA Kit;Human protein disulfide isomerase-associated 5 ELISA Kit;
Catalog
E031528
species
Human
GenePDIA5
SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase A5 (PDIA5). No significant cross-reactivity or interference between Protein Disulfide Isomerase A5 (PDIA5) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.058ng/mL.
Intended UseHuman PDIA5 ELISA Kit allows for the in vitro quantitative determination of PDIA5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A5 (PDIA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A5 (PDIA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase A5 (PDIA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Disulfide Isomerase A5 (PDIA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Protein disulfide-isomerase A5 Protein information
Uniprot ID PDIA5_HUMAN
Uniprot AC Q14554; D3DN95; Q9BV43;
UniGene Hs.477352;
GeneID 10954
KEGG hsa:10954;
Human Protein disulfide-isomerase A5 Protein SEQUENCE
SEQUENCE 519 AA; 59594 MW; 6083FBEB8C019658 CRC64;
MARAGPAWLL LAIWVVLPSW LSSAKVSSLI ERISDPKDLK KLLRTRNNVL
VLYSKSEVAA ENHLRLLSTV AQAVKGQGTI CWVDCGDAES RKLCKKMKVD
LSPKDKKVEL FHYQDGAFHT EYNRAVTFKS IVAFLKDPKG PPLWEEDPGA
KDVVHLDSEK DFRRLLKKEE KPLLIMFYAP WCSMCKRMMP HFQKAATQLR
GHAVLAGMNV YSSEFENIKE EYSVRGFPTI CYFEKGRFLF QYDNYGSTAE
DIVEWLKNPQ PPQPQVPETP WADEGGSVYH LTDEDFDQFV KEHSSVLVMF
HAPWCGHCKK MKPEFEKAAE ALHGEADSSG VLAAVDATVN KALAERFHIS
EFPTLKYFKN GEKYAVPVLR TKKKFLEWMQ NPEAPPPPEP TWEEQQTSVL
HLVGDNFRET LKKKKHTLVM FYAPWCPHCK KVIPHFTATA DAFKDDRKIA
CAAVDCVKDK NQDLCQQEAV KGYPTFHYYH YGKFAEKYDS DRTELGFTNY
IRALREGDHE RLGKKKEEL
UCSC uc003egc.3; human. [Q14554-1];

Category

Human PDIA5 ELISA Kit

Human protein disulfide isomerase-related protein ELISA Kit;Human PDIR ELISA Kit;Human protein disulfide isomerase family A member 5 ELISA Kit;Human protein disulfide-isomerase A5 ELISA Kit;Human protein disulfide isomerase-associated 5 ELISA Kit;