Human PDIA5 ELISA Kit

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  • Alternative name

    Human protein disulfide isomerase-related protein ELISA Kit;Human PDIR ELISA Kit;Human protein disulfide isomerase family A member 5 ELISA Kit;Human protein disulfide-isomerase A5 ELISA Kit;Human protein disulfide isomerase-associated 5 ELISA Kit;

  • Catalog
    E031528
  • species
    Human
  • GenePDIA5
  • Standard CurveHuman PDIA5 ELISA Kit
  • Other Species Mouse Pdia5 ELISA KitBovine PDIA5 ELISA KitRat Pdia5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase A5 (PDIA5). No significant cross-reactivity or interference between Protein Disulfide Isomerase A5 (PDIA5) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.058ng/mL.
  • Intended UseHuman PDIA5 ELISA Kit allows for the in vitro quantitative determination of PDIA5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A5 (PDIA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A5 (PDIA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase A5 (PDIA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Disulfide Isomerase A5 (PDIA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Protein disulfide-isomerase A5 Protein information
  • Uniprot ID PDIA5_HUMAN
  • Uniprot AC Q14554; D3DN95; Q9BV43;
  • UniGene Hs.477352;
  • GeneID 10954
  • KEGG hsa:10954;
  • Human Protein disulfide-isomerase A5 Protein SEQUENCE
  • SEQUENCE 519 AA; 59594 MW; 6083FBEB8C019658 CRC64;

    MARAGPAWLL LAIWVVLPSW LSSAKVSSLI ERISDPKDLK KLLRTRNNVL

    VLYSKSEVAA ENHLRLLSTV AQAVKGQGTI CWVDCGDAES RKLCKKMKVD

    LSPKDKKVEL FHYQDGAFHT EYNRAVTFKS IVAFLKDPKG PPLWEEDPGA

    KDVVHLDSEK DFRRLLKKEE KPLLIMFYAP WCSMCKRMMP HFQKAATQLR

    GHAVLAGMNV YSSEFENIKE EYSVRGFPTI CYFEKGRFLF QYDNYGSTAE

    DIVEWLKNPQ PPQPQVPETP WADEGGSVYH LTDEDFDQFV KEHSSVLVMF

    HAPWCGHCKK MKPEFEKAAE ALHGEADSSG VLAAVDATVN KALAERFHIS

    EFPTLKYFKN GEKYAVPVLR TKKKFLEWMQ NPEAPPPPEP TWEEQQTSVL

    HLVGDNFRET LKKKKHTLVM FYAPWCPHCK KVIPHFTATA DAFKDDRKIA

    CAAVDCVKDK NQDLCQQEAV KGYPTFHYYH YGKFAEKYDS DRTELGFTNY

    IRALREGDHE RLGKKKEEL

  • UCSC uc003egc.3; human. [Q14554-1];


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