Human PSMA5 ELISA Kit

Alternative name
Human macropain zeta chain ELISA Kit;Human multicatalytic endopeptidase complex zeta chain ELISA Kit;Human proteasome zeta chain ELISA Kit;Human PSC5 ELISA Kit;Human ZETA ELISA Kit;Human proteasome subunit alpha 5 ELISA Kit;Human proteasome subunit alpha type-5 ELISA Kit;Human epididymis tissue sperm binding protein Li 11n ELISA Kit;Human macropain subunit zeta ELISA Kit;Human proteasome (prosome, macropain) subunit, alpha type, 5 ELISA Kit;Human proteasome alpha 5 subunit ELISA Kit;Human proteasome component 5 ELISA Kit;Human proteasome subunit zeta ELISA Kit;
Catalog
E031452
species
Human
GenePSMA5
SpecificityThis assay has high sensitivity and excellent specificity for detection of Proteasome Subunit Alpha Type 5 (PSMa5). No significant cross-reactivity or interference between Proteasome Subunit Alpha Type 5 (PSMa5) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.124ng/mL.
Intended UseHuman PSMA5 ELISA Kit allows for the in vitro quantitative determination of PSMA5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteasome Subunit Alpha Type 5 (PSMa5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proteasome Subunit Alpha Type 5 (PSMa5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteasome Subunit Alpha Type 5 (PSMa5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proteasome Subunit Alpha Type 5 (PSMa5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Proteasome subunit alpha type-5 Protein information
Uniprot ID PSA5_HUMAN
Uniprot AC P28066; B2R8F6; B4E2V4; Q3T1C1; Q6IBF7;
UniGene Hs.485246;
GeneID 5686
KEGG hsa:5686;
Human Proteasome subunit alpha type-5 Protein SEQUENCE
SEQUENCE 241 AA; 26411 MW; 5610CDA00469120A CRC64;
MFLTRSEYDR GVNTFSPEGR LFQVEYAIEA IKLGSTAIGI QTSEGVCLAV
EKRITSPLME PSSIEKIVEI DAHIGCAMSG LIADAKTLID KARVETQNHW
FTYNETMTVE SVTQAVSNLA LQFGEEDADP GAMSRPFGVA LLFGGVDEKG
PQLFHMDPSG TFVQCDARAI GSASEGAQSS LQEVYHKSMT LKEAIKSSLI
ILKQVMEEKL NATNIELATV QPGQNFHMFT KEELEEVIKD I
UCSC uc001dxn.4; human. [P28066-1];

Category

Human PSMA5 ELISA Kit