Human PRSS50 ELISA Kit

Alternative name
Human cancer/testis antigen 20 ELISA Kit;Human serine protease 50 ELISA Kit;Human testis-specific protease-like protein 50 ELISA Kit;Human CT20 ELISA Kit;Human TSP50 ELISA Kit;Human protease, serine 50 ELISA Kit;Human probable threonine protease PRSS50 ELISA Kit;Human testes-specific protease 50 ELISA Kit;Human testicular tissue protein Li 210 ELISA Kit;
Catalog
E031401
species
Human
GenePRSS50
SpecificityThis assay has high sensitivity and excellent specificity for detection of Protease, Serine 50 (PRSS50). No significant cross-reactivity or interference between Protease, Serine 50 (PRSS50) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.062ng/mL.
Intended UseHuman PRSS50 ELISA Kit allows for the in vitro quantitative determination of PRSS50 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protease, Serine 50 (PRSS50). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protease, Serine 50 (PRSS50). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protease, Serine 50 (PRSS50), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protease, Serine 50 (PRSS50) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Probable threonine protease PRSS50 Protein information
Uniprot ID TSP50_HUMAN
Uniprot AC Q9UI38;
UniGene Hs.120365;
GeneID 29122
KEGG hsa:29122;
Human Probable threonine protease PRSS50 Protein SEQUENCE
SEQUENCE 385 AA; 43088 MW; F5B39D6E12798AE5 CRC64;
MGRWCQTVAR GQRPRTSAPS RAGALLLLLL LLRSAGCWGA GEAPGALSTA
DPADQSVQCV PKATCPSSRP RLLWQTPTTQ TLPSTTMETQ FPVSEGKVDP
YRSCGFSYEQ DPTLRDPEAV ARRWPWMVSV RANGTHICAG TIIASQWVLT
VAHCLIWRDV IYSVRVGSPW IDQMTQTASD VPVLQVIMHS RYRAQRFWSW
VGQANDIGLL KLKQELKYSN YVRPICLPGT DYVLKDHSRC TVTGWGLSKA
DGMWPQFRTI QEKEVIILNN KECDNFYHNF TKIPTLVQII KSQMMCAEDT
HREKFCYELT GEPLVCSMEG TWYLVGLVSW GAGCQKSEAP PIYLQVSSYQ
HWIWDCLNGQ ALALPAPSRT LLLALPLPLS LLAAL
UCSC uc003cqe.2; human.;

Category

Human PRSS50 ELISA Kit