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Human PRSS12 ELISA Kit

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  • Alternative name

    Human leydin ELISA Kit;Human Motopsin ELISA Kit;Human Serine protease 12 ELISA Kit;Human BSSP-3 ELISA Kit;Human BSSP3 ELISA Kit;Human MRT1 ELISA Kit;Human protease, serine 12 ELISA Kit;Human neurotrypsin ELISA Kit;Human brain-specific serine protease 3 ELISA Kit;Human protease, serine, 12 (neurotrypsin, motopsin) ELISA Kit;
  • Catalog
    E031388
  • species
    Human
  • GenePRSS12
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman PRSS12 ELISA Kit allows for the in vitro quantitative determination of PRSS12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: PRSS12 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PRSS12 antibody and an PRSS12-HRP conjugate. The assay sample and buffer are incubated together with PRSS12-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRSS12 concentration since PRSS12 from samples and PRSS12-HRP conjugate compete for the anti-PRSS12 antibody binding site. Since the number of sites is limited, as more sites are occupied by PRSS12 from the sample, fewer sites are left to bind PRSS12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRSS12 concentration in each sample is interpolated from this standard curve.
  • Human Neurotrypsin Protein information
  • Uniprot ID NETR_HUMAN
  • Uniprot AC P56730; Q9UP16;
  • UniGene Hs.445857;
  • GeneID 8492
  • KEGG hsa:8492;
  • Human Neurotrypsin Protein SEQUENCE

  • SEQUENCE 875 AA; 97067 MW; EB357047C39371BC CRC64;

    MTLARFVLAL MLGALPEVVG FDSVLNDSLH HSHRHSPPAG PHYPYYLPTQ

    QRPPRTRPPP PLPRFPRPPR ALPAQRPHAL QAGHTPRPHP WGCPAGEPWV

    SVTDFGAPCL RWAEVPPFLE RSPPASWAQL RGQRHNFCRS PDGAGRPWCF

    YGDARGKVDW GYCDCRHGSV RLRGGKNEFE GTVEVYASGV WGTVCSSHWD

    DSDASVICHQ LQLGGKGIAK QTPFSGLGLI PIYWSNVRCR GDEENILLCE

    KDIWQGGVCP QKMAAAVTCS FSHGPTFPII RLAGGSSVHE GRVELYHAGQ

    WGTVCDDQWD DADAEVICRQ LGLSGIAKAW HQAYFGEGSG PVMLDEVRCT

    GNELSIEQCP KSSWGEHNCG HKEDAGVSCT PLTDGVIRLA GGKGSHEGRL

    EVYYRGQWGT VCDDGWTELN TYVVCRQLGF KYGKQASANH FEESTGPIWL

    DDVSCSGKET RFLQCSRRQW GRHDCSHRED VSIACYPGGE GHRLSLGFPV

    RLMDGENKKE GRVEVFINGQ WGTICDDGWT DKDAAVICRQ LGYKGPARAR

    TMAYFGEGKG PIHVDNVKCT GNERSLADCI KQDIGRHNCR HSEDAGVICD

    YFGKKASGNS NKESLSSVCG LRLLHRRQKR IIGGKNSLRG GWPWQVSLRL

    KSSHGDGRLL CGATLLSSCW VLTAAHCFKR YGNSTRSYAV RVGDYHTLVP

    EEFEEEIGVQ QIVIHREYRP DRSDYDIALV RLQGPEEQCA RFSSHVLPAC

    LPLWRERPQK TASNCYITGW GDTGRAYSRT LQQAAIPLLP KRFCEERYKG

    RFTGRMLCAG NLHEHKRVDS CQGDSGGPLM CERPGESWVV YGVTSWGYGC

    GVKDSPGVYT KVSAFVPWIK SVTKL

  • UCSC uc003ica.3; human.;



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