Human PRSS1 ELISA Kit

Alternative name
Human beta-trypsin ELISA Kit;Human cationic trypsinogen ELISA Kit;Human Serine protease 1 ELISA Kit;Human Trypsin I ELISA Kit;Human TRP1 ELISA Kit;Human TRY1 ELISA Kit;Human TRYP1 ELISA Kit;Human TRY4 ELISA Kit;Human protease, serine 1 ELISA Kit;Human trypsin-1 ELISA Kit;Human TCR V beta 4.1 ELISA Kit;Human digestive zymogen ELISA Kit;Human nonfunctional trypsin 1 ELISA Kit;Human trypsinogen 1 ELISA Kit;Human trypsinogen A ELISA Kit;
Catalog
E031386
species
Human
GenePRSS1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 U/L.
Intended UseHuman PRSS1 ELISA Kit allows for the in vitro quantitative determination of PRSS1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: PRSS1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PRSS1 antibody and an PRSS1-HRP conjugate. The assay sample and buffer are incubated together with PRSS1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRSS1 concentration since PRSS1 from samples and PRSS1-HRP conjugate compete for the anti-PRSS1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PRSS1 from the sample, fewer sites are left to bind PRSS1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRSS1 concentration in each sample is interpolated from this standard curve.
Human Alpha-trypsin chain 2 Protein information
Uniprot ID TRY1_HUMAN
Uniprot AC Q9HAN4; Q9HAN5; Q9HAN6; Q9HAN7;
UniGene Hs.382212; Hs.449276; Hs.449281;
GeneID 5644
KEGG hsa:5644;
Human Alpha-trypsin chain 2 Protein SEQUENCE
SEQUENCE 247 AA; 26558 MW; DD49A487B8062813 CRC64;
MNPLLILTFV AAALAAPFDD DDKIVGGYNC EENSVPYQVS LNSGYHFCGG
SLINEQWVVS AGHCYKSRIQ VRLGEHNIEV LEGNEQFINA AKIIRHPQYD
RKTLNNDIML IKLSSRAVIN ARVSTISLPT APPATGTKCL ISGWGNTASS
GADYPDELQC LDAPVLSQAK CEASYPGKIT SNMFCVGFLE GGKDSCQGDS
GGPVVCNGQL QGVVSWGDGC AQKNKPGVYT KVYNYVKWIK NTIAANS
UCSC uc003wak.3; human.;

Human PRSS1 ELISA Kit

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