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Alternative name
Proteinase-activated receptor 2 ELISA KIT; Coagulation factor II receptor-like 1 ELISA KIT; G-protein coupled receptor 11 ELISA KIT; Thrombin receptor-like 1Cleaved into the following 2 chains:Proteinase-activated receptor 2, alternate cleaved 1 ELISA KIT; Proteinase-activated receptor 2, alternate cleaved 2F2RL1 ELISA KIT; GPR11 ELISA KIT; PAR2 ELISA KIT; PAR-2 ELISA KIT
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Catalog
E031377
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species
Human
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GenePAR2
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Standard Curve
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Other Species
Mouse PAR2 ELISA Kit
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseHuman PAR2 ELISA Kit allows for the in vitro quantitative determination of PAR2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilySignal Transduction
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Product Description
specificalPrinciple of the Assay: PAR2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PAR2. Standards or samples are then added to the microtiter plate wells and PAR2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PAR2 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PAR2 are added to each well to "sandwich" the PAR2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PAR2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAR2 concentration in each sample is interpolated from this standard curve.
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