-
Alternative name
Proteinase-activated receptor 1 ELISA KIT; Coagulation factor II receptor ELISA KIT; Thrombin receptorF2R ELISA KIT; CF2R ELISA KIT; PAR1 ELISA KIT; TR ELISA KIT; PAR-1 ELISA KIT
-
Catalog
E031376
-
species
Human
-
GenePAR1
-
SpecificityThis assay has high sensitivity and excellent specificity for detection of Protease Activated Receptor 1 (PAR1).
No significant cross-reactivity or interference between Protease Activated Receptor 1 (PAR1) and analogues was observed.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
SensitivityTypically less than 0.059ng/mL.
-
Intended UseHuman PAR1 ELISA Kit allows for the in vitro quantitative determination of PAR1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protease Activated Receptor 1 (PAR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protease Activated Receptor 1 (PAR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protease Activated Receptor 1 (PAR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protease Activated Receptor 1 (PAR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-