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                                    Alternative name
Human sperm histone P2 ELISA Kit;Human sperm protamine P2 ELISA Kit;Human Sperm protamine P4 ELISA Kit;Human P2' ELISA Kit;Human P2'' ELISA Kit;Human Sperm protamine P3 ELISA Kit;Human CT94.2 ELISA Kit;Human protamine 2 ELISA Kit;Human protamine-2 ELISA Kit;Human cancer/testis antigen family 94, member 2 ELISA Kit;Human testicular secretory protein Li 40 ELISA Kit;
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Catalog
E031372
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species
Human
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                                    GenePRM2
                                
                             
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                                    SpecificityThis assay has high sensitivity and excellent specificity for detection of Protamine 2 (PRM2). 
No significant cross-reactivity or interference between Protamine 2 (PRM2) and analogues was observed.
                                
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                                    SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
                                
 
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                                    SensitivityTypically less than 0.057ng/mL.
                                
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                                Intended UseHuman PRM2 ELISA Kit allows for the in vitro quantitative determination of PRM2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
                            
  
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                                    StorageStore the whole ELISA kit at 4℃
                                
  
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                                    Product Description 
 specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protamine 2 (PRM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protamine 2 (PRM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protamine 2 (PRM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protamine 2 (PRM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
                                 
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                                    Human Sperm histone HP3 Protein information
                                
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                                    Uniprot ID
                                    
                                        PRM2_HUMAN                                    	
                                
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                                    Uniprot AC 
                                        P04554; Q6ZMM0;                                    		
                                
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                                    UniGene
                                        Hs.2324;                                    	
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                                    GeneID 
                                    
                                        5620                                    	
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                                     KEGG
                                        hsa:5620;                                    
 
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                                        Human Sperm histone HP3 Protein SEQUENCE 
                                    
                                
 
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                                SEQUENCE   102 AA;  13051 MW;  CBB8D6F2396F2F9C CRC64;
 MVRYRVRSLS ERSHEVYRQQ LHGQEQGHHG QEEQGLSPEH VEVYERTHGQ
SHYRRRHCSR
 RRLHRIHRRQ HRSCRRRKRR SCRHRRRHRR GCRTRKRTCR
RH
 
                                
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                                    UCSC
                                        uc032drg.2; human. [P04554-1];                                    
 
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