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Alternative name
Human sperm histone P2 ELISA Kit;Human sperm protamine P2 ELISA Kit;Human Sperm protamine P4 ELISA Kit;Human P2' ELISA Kit;Human P2'' ELISA Kit;Human Sperm protamine P3 ELISA Kit;Human CT94.2 ELISA Kit;Human protamine 2 ELISA Kit;Human protamine-2 ELISA Kit;Human cancer/testis antigen family 94, member 2 ELISA Kit;Human testicular secretory protein Li 40 ELISA Kit;
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Catalog
E031372
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species
Human
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GenePRM2
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Standard Curve
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Other Species
Mouse Prm2 ELISA KitBovine PRM2 ELISA KitPorcine PRM2 ELISA KitRat Prm2 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Protamine 2 (PRM2).
No significant cross-reactivity or interference between Protamine 2 (PRM2) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.057ng/mL.
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Intended UseHuman PRM2 ELISA Kit allows for the in vitro quantitative determination of PRM2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protamine 2 (PRM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protamine 2 (PRM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protamine 2 (PRM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protamine 2 (PRM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Human Sperm histone HP3 Protein information
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Uniprot ID
PRM2_HUMAN
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Uniprot AC
P04554; Q6ZMM0;
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UniGene
Hs.2324;
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GeneID
5620
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KEGG
hsa:5620;
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Human Sperm histone HP3 Protein SEQUENCE
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SEQUENCE 102 AA; 13051 MW; CBB8D6F2396F2F9C CRC64;
MVRYRVRSLS ERSHEVYRQQ LHGQEQGHHG QEEQGLSPEH VEVYERTHGQ
SHYRRRHCSR
RRLHRIHRRQ HRSCRRRKRR SCRHRRRHRR GCRTRKRTCR
RH
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UCSC
uc032drg.2; human. [P04554-1];
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