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Alternative name
AncA ELISA KIT; AncAancA ELISA KIT; KNP414_00655 ELISA KIT
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Catalog
E003130
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species
Human
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GeneANCA
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Standard Curve
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Other Species
Human C-ANCA / PR3 IgG ELISA KitHuman MPO-ANCA IgG ELISA KitHuman MPO ANCA IgG ELISA KitHuman P-ANCA / MPO IgG ELISA KitHuman PR3 ANCA ELISA KitHuman PR3-ANCA ELISA KitMouse ANCA ELISA KitMouse C-ANCA / PR3 IgG ELISA KitMouse MPO-ANCA IgG ELISA KitMouse P-ANCA / MPO IgG ELISA KitMouse PR3-ANCA ELISA Kit
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SpecificityNo cross-reaction with other factors.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.6 U/ml.
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Intended UseHuman ANCA ELISA Kit allows for the in vitro quantitative determination of ANCA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ANCA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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