Human PTGS2 ELISA Kit

Alternative name
Human Cyclooxygenase-2 ELISA Kit;Human COX-2 ELISA Kit;Human PHS II ELISA Kit;Human prostaglandin H2 synthase 2 ELISA Kit;Human PGH synthase 2 ELISA Kit;Human PGHS-2 ELISA Kit;Human prostaglandin-endoperoxide synthase 2 ELISA Kit;Human COX2 ELISA Kit;Human GRIPGHS ELISA Kit;Human PGG/HS ELISA Kit;Human PHS-2 ELISA Kit;Human hCox-2 ELISA Kit;Human prostaglandin G/H synthase 2 ELISA Kit;Human cyclooxygenase 2 ELISA Kit;Human cyclooxygenase 2b ELISA Kit;Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) ELISA Kit;
Catalog
E031292
species
Human
GenePTGS2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Prostaglandin Endoperoxide Synthase 2 (PTGS2). No significant cross-reactivity or interference between Prostaglandin Endoperoxide Synthase 2 (PTGS2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.119ng/mL.
Intended UseHuman PTGS2 ELISA Kit allows for the in vitro quantitative determination of PTGS2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin Endoperoxide Synthase 2 (PTGS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostaglandin Endoperoxide Synthase 2 (PTGS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostaglandin Endoperoxide Synthase 2 (PTGS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin Endoperoxide Synthase 2 (PTGS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Prostaglandin G/H synthase 2 Protein information
Uniprot ID PGH2_HUMAN
Uniprot AC P35354; A8K802; Q16876;
UniGene Hs.196384;
GeneID 5743
KEGG hsa:5743;
Human Prostaglandin G/H synthase 2 Protein SEQUENCE
SEQUENCE 604 AA; 68996 MW; 72FBD699F6128519 CRC64;
MLARALLLCA VLALSHTANP CCSHPCQNRG VCMSVGFDQY KCDCTRTGFY
GENCSTPEFL TRIKLFLKPT PNTVHYILTH FKGFWNVVNN IPFLRNAIMS
YVLTSRSHLI DSPPTYNADY GYKSWEAFSN LSYYTRALPP VPDDCPTPLG
VKGKKQLPDS NEIVEKLLLR RKFIPDPQGS NMMFAFFAQH FTHQFFKTDH
KRGPAFTNGL GHGVDLNHIY GETLARQRKL RLFKDGKMKY QIIDGEMYPP
TVKDTQAEMI YPPQVPEHLR FAVGQEVFGL VPGLMMYATI WLREHNRVCD
VLKQEHPEWG DEQLFQTSRL ILIGETIKIV IEDYVQHLSG YHFKLKFDPE
LLFNKQFQYQ NRIAAEFNTL YHWHPLLPDT FQIHDQKYNY QQFIYNNSIL
LEHGITQFVE SFTRQIAGRV AGGRNVPPAV QKVSQASIDQ SRQMKYQSFN
EYRKRFMLKP YESFEELTGE KEMSAELEAL YGDIDAVELY PALLVEKPRP
DAIFGETMVE VGAPFSLKGL MGNVICSPAY WKPSTFGGEV GFQIINTASI
QSLICNNVKG CPFTSFSVPD PELIKTVTIN ASSSRSGLDD INPTVLLKER
STEL
UCSC uc001gsb.4; human.;

Category

Human PTGS2 ELISA Kit