Human PML ELISA Kit

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  • Alternative name

    Human promyelocytic leukemia protein ELISA Kit;Human RING finger protein 71 ELISA Kit;Human tripartite motif-containing protein 19 ELISA Kit;Human MYL ELISA Kit;Human PP8675 ELISA Kit;Human RNF71 ELISA Kit;Human TRIM19 ELISA Kit;Human promyelocytic leukemia ELISA Kit;Human protein PML ELISA Kit;Human PML/RARA fusion ELISA Kit;Human probable transcription factor PML ELISA Kit;Human promyelocytic leukemia, inducer of ELISA Kit;Human tripartite motif protein TRIM19 ELISA Kit;

  • Catalog
    E031183
  • species
    Human
  • GenePML
  • Standard CurveHuman PML ELISA Kit
  • Other Species Mouse Pml ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Promyelocytic Leukemia Protein (PML). No significant cross-reactivity or interference between Promyelocytic Leukemia Protein (PML) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.066ng/mL.
  • Intended UseHuman PML ELISA Kit allows for the in vitro quantitative determination of PML , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Promyelocytic Leukemia Protein (PML). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Promyelocytic Leukemia Protein (PML). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Promyelocytic Leukemia Protein (PML), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Promyelocytic Leukemia Protein (PML) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Protein PML Protein information
  • Uniprot ID PML_HUMAN
  • Uniprot AC Q9BZY0; Q9BZY2; Q9BZY3;
  • UniGene Hs.526464; Hs.654583;
  • GeneID 5371
  • KEGG hsa:5371;
  • Human Protein PML Protein SEQUENCE
  • SEQUENCE 882 AA; 97551 MW; D50968A977E34287 CRC64;

    MEPAPARSPR PQQDPARPQE PTMPPPETPS EGRQPSPSPS PTERAPASEE

    EFQFLRCQQC QAEAKCPKLL PCLHTLCSGC LEASGMQCPI CQAPWPLGAD

    TPALDNVFFE SLQRRLSVYR QIVDAQAVCT RCKESADFWC FECEQLLCAK

    CFEAHQWFLK HEARPLAELR NQSVREFLDG TRKTNNIFCS NPNHRTPTLT

    SIYCRGCSKP LCCSCALLDS SHSELKCDIS AEIQQRQEEL DAMTQALQEQ

    DSAFGAVHAQ MHAAVGQLGR ARAETEELIR ERVRQVVAHV RAQERELLEA

    VDARYQRDYE EMASRLGRLD AVLQRIRTGS ALVQRMKCYA SDQEVLDMHG

    FLRQALCRLR QEEPQSLQAA VRTDGFDEFK VRLQDLSSCI TQGKDAAVSK

    KASPEAASTP RDPIDVDLPE EAERVKAQVQ ALGLAEAQPM AVVQSVPGAH

    PVPVYAFSIK GPSYGEDVSN TTTAQKRKCS QTQCPRKVIK MESEEGKEAR

    LARSSPEQPR PSTSKAVSPP HLDGPPSPRS PVIGSEVFLP NSNHVASGAG

    EAEERVVVIS SSEDSDAENS SSRELDDSSS ESSDLQLEGP STLRVLDENL

    ADPQAEDRPL VFFDLKIDNE TQKISQLAAV NRESKFRVVI QPEAFFSIYS

    KAVSLEVGLQ HFLSFLSSMR RPILACYKLW GPGLPNFFRA LEDINRLWEF

    QEAISGFLAA LPLIRERVPG ASSFKLKNLA QTYLARNMSE RSAMAAVLAM

    RDLCRLLEVS PGPQLAQHVY PFSSLQCFAS LQPLVQAAVL PRAEARLLAL

    HNVSFMELLS AHRRDRQGGL KKYSRYLSLQ TTTLPPAQPA FNLQALGTYF

    EGLLEGPALA RAEGVSTPLA GRGLAERASQ QS

  • UCSC uc002awk.4; human. [P29590-1];


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