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Alternative name
Human MAP ELISA Kit;Human motilin ELISA Kit;Human promotilin ELISA Kit;Human prepromotilin ELISA Kit;
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Catalog
E031181
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species
Human
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GeneMLN
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 9.4pg/ml
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Intended UseHuman MLN ELISA Kit allows for the in vitro quantitative determination of MLN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human MLN antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human MLN antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MLN amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human MLN can be calculated.
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Human Motilin-associated peptide Protein information
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Uniprot ID
MOTI_HUMAN
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Uniprot AC
P12872; B7ZLR7; E9PDN2; J3KN51; Q2M1L2; Q5T975; Q6NSY7;
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UniGene
Hs.2813;
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GeneID
4295
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KEGG
hsa:4295;
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Human Motilin-associated peptide Protein SEQUENCE
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SEQUENCE 115 AA; 12920 MW; 30D4BB59B2F42783 CRC64;
MVSRKAVAAL LVVHVAAMLA SQTEAFVPIF TYGELQRMQE KERNKGQKKS
LSVWQRSGEE
GPVDPAEPIR EEENEMIKLT APLEIGMRMN SRQLEKYPAT
LEGLLSEMLP QHAAK
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UCSC
uc003off.2; human. [P12872-1];
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