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Human P4HA3 ELISA Kit

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  • Alternative name

    Human 4-PH alpha-3 ELISA Kit;Human Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3 ELISA Kit;Human prolyl 4-hydroxylase subunit alpha 3 ELISA Kit;Human prolyl 4-hydroxylase subunit alpha-3 ELISA Kit;Human C-P4H alpha III ELISA Kit;Human collagen prolyl 4-hydroxylase alpha(III) ELISA Kit;Human procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III ELISA Kit;Human prolyl 4-hydroxylase, alpha polypeptide III ELISA Kit;
  • Catalog
    E031167
  • species
    Human
  • GeneP4HA3
  • Standard CurveHuman P4HA3 ELISA Kit
  • Other Species Mouse P4ha3 ELISA KitBovine P4HA3 ELISA KitRat P4ha3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3). No significant cross-reactivity or interference between Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.066ng/mL.
  • Intended UseHuman P4HA3 ELISA Kit allows for the in vitro quantitative determination of P4HA3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Prolyl 4-hydroxylase subunit alpha-3 Protein information
  • Uniprot ID P4HA3_HUMAN
  • Uniprot AC Q7Z4N8; A0AV13; B4DUD3; Q5EBL3; Q5JPA9;
  • UniGene Hs.660541;
  • GeneID 283208
  • KEGG hsa:283208;
  • Human Prolyl 4-hydroxylase subunit alpha-3 Protein SEQUENCE

  • SEQUENCE 544 AA; 61126 MW; 1C88C168A268572F CRC64;

    MGPGARLAAL LAVLALGTGD PERAAARGDT FSALTSVARA LAPERRLLGL

    LRRYLRGEEA RLRDLTRFYD KVLSLHEDST TPVANPLLAF TLIKRLQSDW

    RNVVHSLEAS ENIRALKDGY EKVEQDLPAF EDLEGAARAL MRLQDVYMLN

    VKGLARGVFQ RVTGSAITDL YSPKRLFSLT GDDCFQVGKV AYDMGDYYHA

    IPWLEEAVSL FRGSYGEWKT EDEASLEDAL DHLAFAYFRA GNVSCALSLS

    REFLLYSPDN KRMARNVLKY ERLLAESPNH VVAEAVIQRP NIPHLQTRDT

    YEGLCQTLGS QPTLYQIPSL YCSYETNSNA YLLLQPIRKE VIHLEPYIAL

    YHDFVSDSEA QKIRELAEPW LQRSVVASGE KQLQVEYRIS KSAWLKDTVD

    PKLVTLNHRI AALTGLDVRP PYAEYLQVVN YGIGGHYEPH FDHATSPSSP

    LYRMKSGNRV ATFMIYLSSV EAGGATAFIY ANLSVPVVRN AALFWWNLHR

    SGEGDSDTLH AGCPVLVGDK WVANKWIHEY GQEFRRPCSS SPED

  • UCSC uc001ouz.5; human. [Q7Z4N8-1];


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