Human P4HA2 ELISA Kit

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  • Alternative name

    Human 4-PH alpha-2 ELISA Kit;Human procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2 ELISA Kit;Human MYP25 ELISA Kit;Human prolyl 4-hydroxylase subunit alpha 2 ELISA Kit;Human prolyl 4-hydroxylase subunit alpha-2 ELISA Kit;Human 4-PH alpha 2 ELISA Kit;Human C-P4Halpha(II) ELISA Kit;Human collagen prolyl 4-hydroxylase alpha(II) ELISA Kit;Human procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II ELISA Kit;Human prolyl 4-hydroxylase, alpha polypeptide II ELISA Kit;

  • Catalog
    E031165
  • species
    Human
  • GeneP4HA2
  • Standard CurveHuman P4HA2 ELISA Kit
  • Other Species Mouse P4ha2 ELISA KitChicken P4HA2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Prolyl-4-Hydroxylase Alpha Polypeptide II (P4Ha2). No significant cross-reactivity or interference between Prolyl-4-Hydroxylase Alpha Polypeptide II (P4Ha2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.063ng/mL.
  • Intended UseHuman P4HA2 ELISA Kit allows for the in vitro quantitative determination of P4HA2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide II (P4Ha2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide II (P4Ha2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolyl-4-Hydroxylase Alpha Polypeptide II (P4Ha2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prolyl-4-Hydroxylase Alpha Polypeptide II (P4Ha2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Prolyl 4-hydroxylase subunit alpha-2 Protein information
  • Uniprot ID P4HA2_HUMAN
  • Uniprot AC O15460; D3DQ85; D3DQ86; Q8WWN0;
  • UniGene Hs.519568; Hs.659712;
  • GeneID 8974
  • KEGG hsa:8974;
  • Human Prolyl 4-hydroxylase subunit alpha-2 Protein SEQUENCE
  • SEQUENCE 535 AA; 60902 MW; FD04467B098F63CF CRC64;

    MKLWVSALLM AWFGVLSCVQ AEFFTSIGHM TDLIYAEKEL VQSLKEYILV

    EEAKLSKIKS WANKMEALTS KSAADAEGYL AHPVNAYKLV KRLNTDWPAL

    EDLVLQDSAA GFIANLSVQR QFFPTDEDEI GAAKALMRLQ DTYRLDPGTI

    SRGELPGTKY QAMLSVDDCF GMGRSAYNEG DYYHTVLWME QVLKQLDAGE

    EATTTKSQVL DYLSYAVFQL GDLHRALELT RRLLSLDPSH ERAGGNLRYF

    EQLLEEEREK TLTNQTEAEL ATPEGIYERP VDYLPERDVY ESLCRGEGVK

    LTPRRQKRLF CRYHHGNRAP QLLIAPFKEE DEWDSPHIVR YYDVMSDEEI

    ERIKEIAKPK LARATVRDPK TGVLTVASYR VSKSSWLEED DDPVVARVNR

    RMQHITGLTV KTAELLQVAN YGVGGQYEPH FDFSRNDERD TFKHLGTGNR

    VATFLNYMSD VEAGGATVFP DLGAAIWPKK GTAVFWYNLL RSGEGDYRTR

    HAACPVLVGC KWVSNKWFHE RGQEFLRPCG STEVD

  • UCSC uc003kwg.4; human. [O15460-1];


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