Human P4HB ELISA Kit

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  • Alternative name

    Human PDI ELISA Kit;Human cellular thyroid hormone-binding protein ELISA Kit;Human prolyl 4-hydroxylase subunit beta ELISA Kit;Human p55 ELISA Kit;Human ERBA2L ELISA Kit;Human PDIA1 ELISA Kit;Human PO4DB ELISA Kit;Human CLCRP1 ELISA Kit;Human DSI ELISA Kit;Human GIT ELISA Kit;Human P4Hbeta ELISA Kit;Human PHDB ELISA Kit;Human PO4HB ELISA Kit;Human PROHB ELISA Kit;Human protein disulfide-isomerase ELISA Kit;Human collagen prolyl 4-hydroxylase beta ELISA Kit;Human glutathione-insulin transhydrogenase ELISA Kit;Human procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide ELISA Kit;Human prolyl 4-hydroxylase, beta polypeptide ELISA Kit;Human protein disulfide isomerase family A, member 1 ELISA Kit;Human protein disulfide isomerase-associated 1 ELISA Kit;Human protein disulfide isomerase/oxidoreductase ELISA Kit;Human protocollagen hydroxylase ELISA Kit;Human testicular secretory protein Li 32 ELISA Kit;Human thyroid hormone-binding protein p55 ELISA Kit;

  • Catalog
    E031157
  • species
    Human
  • GeneP4HB
  • Other Species Mouse P4HB ELISA KitChicken P4HB ELISA KitHamster P4HB ELISA KitRabbit P4HB ELISA KitRat P4hb ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human PDI. No significant cross-reactivity or interference between human PDI and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human PDI is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman P4HB ELISA Kit allows for the in vitro quantitative determination of P4HB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDI is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDI bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Protein disulfide-isomerase Protein information
  • Uniprot ID PDIA1_HUMAN
  • Uniprot AC P07237; B2RDQ2; P30037; P32079; Q15205; Q6LDE5;
  • UniGene Hs.464336;
  • GeneID 5034
  • KEGG hsa:5034;
  • Human Protein disulfide-isomerase Protein SEQUENCE
  • SEQUENCE 508 AA; 57116 MW; 906CE6D9900B8FCE CRC64;

    MLRRALLCLA VAALVRADAP EEEDHVLVLR KSNFAEALAA HKYLLVEFYA

    PWCGHCKALA PEYAKAAGKL KAEGSEIRLA KVDATEESDL AQQYGVRGYP

    TIKFFRNGDT ASPKEYTAGR EADDIVNWLK KRTGPAATTL PDGAAAESLV

    ESSEVAVIGF FKDVESDSAK QFLQAAEAID DIPFGITSNS DVFSKYQLDK

    DGVVLFKKFD EGRNNFEGEV TKENLLDFIK HNQLPLVIEF TEQTAPKIFG

    GEIKTHILLF LPKSVSDYDG KLSNFKTAAE SFKGKILFIF IDSDHTDNQR

    ILEFFGLKKE ECPAVRLITL EEEMTKYKPE SEELTAERIT EFCHRFLEGK

    IKPHLMSQEL PEDWDKQPVK VLVGKNFEDV AFDEKKNVFV EFYAPWCGHC

    KQLAPIWDKL GETYKDHENI VIAKMDSTAN EVEAVKVHSF PTLKFFPASA

    DRTVIDYNGE RTLDGFKKFL ESGGQDGAGD DDDLEDLEEA EEPDMEEDDD

    QKAVKDEL

  • UCSC uc002kbn.2; human.;


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