Human PCNA ELISA Kit

Alternative name
Human PCNA ELISA Kit;Human cyclin ELISA Kit;Human ATLD2 ELISA Kit;Human proliferating cell nuclear antigen ELISA Kit;Human DNA polymerase delta auxiliary protein ELISA Kit;
Catalog
E031094
species
Human
GenePCNA
Other Species Mouse Pcna ELISA Kit Bovine PCNA ELISA Kit Chicken PCNA ELISA Kit Hamster PCNA ELISA Kit Monkey PCNA ELISA Kit Rat Pcna ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of human PCNA. No significant cross-reactivity or interference between human PCNA and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human PCNA is typically less than 19.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman PCNA ELISA Kit allows for the in vitro quantitative determination of PCNA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPriciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PCNA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCNA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCNA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCNA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Proliferating cell nuclear antigen Protein information
Uniprot ID PCNA_HUMAN
Uniprot AC P12004; B2R897; D3DW02;
UniGene Hs.147433; Hs.744934;
GeneID 5111
KEGG hsa:5111;
Human Proliferating cell nuclear antigen Protein SEQUENCE
SEQUENCE 261 AA; 28769 MW; E6F08E7EDBC48B00 CRC64;
MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQL
TLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA
LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD
LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN
EPVQLTFALR YLNFFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY
LAPKIEDEEG S
UCSC uc002wlp.4; human.;

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Human PCNA ELISA Kit

Human PCNA ELISA Kit;Human cyclin ELISA Kit;Human ATLD2 ELISA Kit;Human proliferating cell nuclear antigen ELISA Kit;Human DNA polymerase delta auxiliary protein ELISA Kit;