Human PRL ELISA Kit

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  • Alternative name

    Human PRL ELISA Kit;Human GHA1 ELISA Kit;Human prolactin ELISA Kit;Human decidual prolactin ELISA Kit;Human growth hormone A1 ELISA Kit;

  • Catalog
    E031069
  • species
    Human
  • GenePRL
  • Other Species Human PRL/LTH ELISA KitMouse Prl ELISA KitMouse, Rat PRL ELISA KitMouse, Rat PRL ELISA KitMouse PRL/LTH ELISA KitBovine PRL ELISA KitGoat PRL ELISA KitChicken PRL ELISA KitHorse PRL ELISA KitTurkey PRL ELISA KitPorcine PRL ELISA KitRabbit PRL ELISA KitRat Prl ELISA KitSheep PRL ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PRL. No significant cross-reactivity or interference between Human PRL and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity50 uIU/ml
  • Intended UseHuman PRL ELISA Kit allows for the in vitro quantitative determination of PRL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Introduction: Prolactin (PRL) or Luteotropic hormone (LTH) is a peptide hormone discovered by Dr. Henry Friesen, primarily associated with lactation. In breastfeeding, the act of an infant suckling the nipple stimulates the production of prolactin, which fills the breast with milk via a process called lactogenesis, in preparation for the next feed. Oxytocin, another hormone, is also released, which triggers milk let-down. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PRL. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated monoclonal antibody preparation specific for PRL and incubated. Then substrate solutions are added to each well. Only those wells that contain PRL, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PRL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Prolactin Protein information
  • Uniprot ID PRL_HUMAN
  • Uniprot AC P01236; Q15199; Q92996;
  • UniGene Hs.1905; Hs.735971;
  • GeneID 5617
  • KEGG hsa:5617;
  • Human Prolactin Protein SEQUENCE
  • SEQUENCE 227 AA; 25876 MW; 952BBA1B6A955527 CRC64;

    MNIKGSPWKG SLLLLLVSNL LLCQSVAPLP ICPGGAARCQ VTLRDLFDRA

    VVLSHYIHNL SSEMFSEFDK RYTHGRGFIT KAINSCHTSS LATPEDKEQA

    QQMNQKDFLS LIVSILRSWN EPLYHLVTEV RGMQEAPEAI LSKAVEIEEQ

    TKRLLEGMEL IVSQVHPETK ENEIYPVWSG LPSLQMADEE SRLSAYYNLL

    HCLRRDSHKI DNYLKLLKCR IIHNNNC





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