Intended UseHuman PFN2 ELISA Kit allows for the in vitro quantitative determination of PFN2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PFN2. During the reaction,Human PFN2 in the sample or standard competes with a fixed amount of biotin-labeled Human PFN2 for sites on a pre-coated Monoclonal antibody specific to Human PFN2. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human PFN2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Profilin-2 Protein information
P35080; B2R4C8; D3DNI4; Q4VBQ4; Q8WVF9; Q9HBK2;