Human PFN1 ELISA Kit

Alternative name
Human epididymis tissue protein Li 184a ELISA Kit;Human profilin I ELISA Kit;Human ALS18 ELISA Kit;Human profilin 1 ELISA Kit;Human profilin-1 ELISA Kit;
Catalog
E030943
species
Human
GenePFN1
SpecificityThis assay has high sensitivity and excellent specificity for detection of human profilin. No significant cross-reactivity or interference between human profilin and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human profilin is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
Intended UseHuman PFN1 ELISA Kit allows for the in vitro quantitative determination of PFN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for profilin 1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any profilin 1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for profilin 1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of profilin 1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Profilin-1 Protein information
Uniprot ID PROF1_HUMAN
Uniprot AC P07737; Q53Y44;
UniGene Hs.494691;
GeneID 5216
KEGG hsa:5216;
Human Profilin-1 Protein SEQUENCE
SEQUENCE 140 AA; 15054 MW; F725119E55A289EB CRC64;
MAGWNAYIDN LMADGTCQDA AIVGYKDSPS VWAAVPGKTF VNITPAEVGV
LVGKDRSSFY VNGLTLGGQK CSVIRDSLLQ DGEFSMDLRT KSTGGAPTFN
VTVTKTDKTL VLLMGKEGVH GGLINKKCYE MASHLRRSQY
UCSC uc002gaa.5; human.;

Human PFN1 ELISA Kit

Human epididymis tissue protein Li 184a ELISA Kit;Human profilin I ELISA Kit;Human ALS18 ELISA Kit;Human profilin 1 ELISA Kit;Human profilin-1 ELISA Kit;

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