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Human PLOD1 ELISA Kit

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  • Alternative name

    Human Lysyl hydroxylase 1 ELISA Kit;Human LH1 ELISA Kit;Human LLH ELISA Kit;Human PLOD ELISA Kit;Human EDS6 ELISA Kit;Human LH ELISA Kit;Human procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 ELISA Kit;Human lysine hydroxylase ELISA Kit;Human lysyl hydroxlase 1 ELISA Kit;Human procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 ELISA Kit;
  • Catalog
    E030918
  • species
    Human
  • GenePLOD1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 (PLOD1). No significant cross-reactivity or interference between Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 (PLOD1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.066ng/mL.
  • Intended UseHuman PLOD1 ELISA Kit allows for the in vitro quantitative determination of PLOD1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 (PLOD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 (PLOD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 (PLOD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 (PLOD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 Protein information
  • Uniprot ID PLOD1_HUMAN
  • Uniprot AC Q02809; B4DR87; Q96AV9; Q9H132;
  • UniGene Hs.75093;
  • GeneID 5351
  • KEGG hsa:5351;
  • Human Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 Protein SEQUENCE

  • SEQUENCE 727 AA; 83550 MW; 6C3E0C11B15D598C CRC64;

    MRPLLLLALL GWLLLAEAKG DAKPEDNLLV LTVATKETEG FRRFKRSAQF

    FNYKIQALGL GEDWNVEKGT SAGGGQKVRL LKKALEKHAD KEDLVILFAD

    SYDVLFASGP RELLKKFRQA RSQVVFSAEE LIYPDRRLET KYPVVSDGKR

    FLGSGGFIGY APNLSKLVAE WEGQDSDSDQ LFYTKIFLDP EKREQINITL

    DHRCRIFQNL DGALDEVVLK FEMGHVRARN LAYDTLPVLI HGNGPTKLQL

    NYLGNYIPRF WTFETGCTVC DEGLRSLKGI GDEALPTVLV GVFIEQPTPF

    VSLFFQRLLR LHYPQKHMRL FIHNHEQHHK AQVEEFLAQH GSEYQSVKLV

    GPEVRMANAD ARNMGADLCR QDRSCTYYFS VDADVALTEP NSLRLLIQQN

    KNVIAPLMTR HGRLWSNFWG ALSADGYYAR SEDYVDIVQG RRVGVWNVPY

    ISNIYLIKGS ALRGELQSSD LFHHSKLDPD MAFCANIRQQ DVFMFLTNRH

    TLGHLLSLDS YRTTHLHNDL WEVFSNPEDW KEKYIHQNYT KALAGKLVET

    PCPDVYWFPI FTEVACDELV EEMEHFGQWS LGNNKDNRIQ GGYENVPTID

    IHMNQIGFER EWHKFLLEYI APMTEKLYPG YYTRAQFDLA FVVRYKPDEQ

    PSLMPHHDAS TFTINIALNR VGVDYEGGGC RFLRYNCSIR APRKGWTLMH

    PGRLTHYHEG LPTTRGTRYI AVSFVDP

  • UCSC uc001atm.4; human. [Q02809-1];



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