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Alternative name
Charged multivesicular body protein 1a ELISA KIT; Chromatin-modifying protein 1a ELISA KIT; CHMP1a ELISA KIT; Vacuolar protein sorting-associated protein 46-1 ELISA KIT; Vps46-1 ELISA KIT; hVps46-1CHMP1A ELISA KIT; ELISA KIT; CHMP1a ELISA KIT; Vps46-1 ELISA KIT; hVps46-1 ELISA KIT
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Catalog
E030908
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species
Human
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GenePCIII
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Standard Curve
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Procollagen III (PCIII).
No significant cross-reactivity or interference between Procollagen III (PCIII) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.067ng/mL.
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Intended UseHuman PCIII ELISA Kit allows for the in vitro quantitative determination of PCIII , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen III (PCIII). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Procollagen III (PCIII). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen III (PCIII), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen III (PCIII) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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