Intended UseHuman PINP ELISA Kit allows for the in vitro quantitative determination of PINP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses||The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PINP in human serum, plasma and other biological fluids..
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PINP. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PINP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PINP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PINP in the samples is then determined by comparing the O.D. of the samples to the standard curve.