Sensitivity0.156 ng/mL (mean of 6 independent assays).
Intended UseHuman WHSC1 ELISA Kit allows for the in vitro quantitative determination of WHSC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: For the quantitative detection of Human Probable histone-lysine N-methyltransferase NSD2 (WHSC1) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitative WHSC1 in Human serum, plasma. An antibody specific for WHSC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WHSC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for WHSC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WHSC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.