Human MKRN3 ELISA Kit

Alternative name
Human RING finger protein 63 ELISA Kit;Human RING-type E3 ubiquitin transferase makorin-3 ELISA Kit;Human zinc finger protein 127 ELISA Kit;Human D15S9 ELISA Kit;Human RNF63 ELISA Kit;Human ZNF127 ELISA Kit;Human CPPB2 ELISA Kit;Human ZFP127 ELISA Kit;Human makorin ring finger protein 3 ELISA Kit;Human probable E3 ubiquitin-protein ligase makorin-3 ELISA Kit;Human RING-type E3 ubiquitin transferase makorin-3 ELISA Kit;
Catalog
E030768
species
Human
GeneMKRN3
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human MKRN3. No significant cross-reactivity or interference between Human MKRN3 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity7.8 pg/mL (mean of 6 independent assays).
Intended UseHuman MKRN3 ELISA Kit allows for the in vitro quantitative determination of MKRN3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: For the quantitative detection of Human Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) concentration in serum, plasma, tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitative MKRN3 in Human serum, plasma, tissue homogenates, cell lysates. An antibody specific for MKRN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MKRN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for MKRN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MKRN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Probable E3 ubiquitin-protein ligase makorin-3 Protein information
Uniprot ID MKRN3_HUMAN
Uniprot AC Q13064;
UniGene Hs.679587; Hs.72964;
GeneID 7681
KEGG hsa:7681;
Human Probable E3 ubiquitin-protein ligase makorin-3 Protein SEQUENCE
SEQUENCE 507 AA; 55645 MW; 2EDBA91190F3A292 CRC64;
MEEPAAPSEA HEAAGAQAGA EAAREGVSGP DLPVCEPSGE SAAPDSALPH
AARGWAPFPV APVPAHLRRG GLRPAPASGG GAWPSPLPSR SSGIWTKQII
CRYYIHGQCK EGENCRYSHD LSGRKMATEG GVSPPGASAG GGPSTAAHIE
PPTQEVAEAP PAASSLSLPV IGSAAERGFF EAERDNADRG AAGGAGVESW
ADAIEFVPGQ PYRGRWVASA PEAPLQSSET ERKQMAVGSG LRFCYYASRG
VCFRGESCMY LHGDICDMCG LQTLHPMDAA QREEHMRACI EAHEKDMELS
FAVQRGMDKV CGICMEVVYE KANPNDRRFG ILSNCNHSFC IRCIRRWRSA
RQFENRIVKS CPQCRVTSEL VIPSEFWVEE EEEKQKLIQQ YKEAMSNKAC
RYFAEGRGNC PFGDTCFYKH EYPEGWGDEP PGPGGGSFSA YWHQLVEPVR
MGEGNMLYKS IKKELVVLRL ASLLFKRFLS LRDELPFSED QWDLLHYELE
EYFNLIL
UCSC uc001ywh.5; human.;

Category

Human MKRN3 ELISA Kit