Human DDX4 ELISA Kit

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  • Alternative name

    Human DEAD box protein 4 ELISA Kit;Human vasa homolog ELISA Kit;Human VASA ELISA Kit;Human DEAD-box helicase 4 ELISA Kit;Human probable ATP-dependent RNA helicase DDX4 ELISA Kit;Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 ELISA Kit;Human DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 ELISA Kit;

  • Catalog
    E030717
  • species
    Human
  • GeneDDX4
  • Other Species Mouse Ddx4 ELISA KitBovine DDX4 ELISA KitMonkey DDX4 ELISA KitPorcine DDX4 ELISA KitRat Ddx4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human DDX4. No significant cross-reactivity or interference between Human DDX4 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe limit of detection of Human DDX4 defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 0.1 ng/mL (mean of 6 independent assays).
  • Intended UseHuman DDX4 ELISA Kit allows for the in vitro quantitative determination of DDX4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitative DDX4 in Human serum, plasma. An antibody specific for DDX4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DDX4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for DDX4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DDX4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Probable ATP-dependent RNA helicase DDX4 Protein information
  • Uniprot ID DDX4_HUMAN
  • Uniprot AC Q9NYB1;
  • UniGene Hs.223581;
  • GeneID 54514
  • KEGG hsa:54514;
  • Human Probable ATP-dependent RNA helicase DDX4 Protein SEQUENCE
  • SEQUENCE 724 AA; 79308 MW; 6D313DD98B177067 CRC64;

    MGDEDWEAEI NPHMSSYVPI FEKDRYSGEN GDNFNRTPAS SSEMDDGPSR

    RDHFMKSGFA SGRNFGNRDA GECNKRDNTS TMGGFGVGKS FGNRGFSNSR

    FEDGDSSGFW RESSNDCEDN PTRNRGFSKR GGYRDGNNSE ASGPYRRGGR

    GSFRGCRGGF GLGSPNNDLD PDECMQRTGG LFGSRRPVLS GTGNGDTSQS

    RSGSGSERGG YKGLNEEVIT GSGKNSWKSE AEGGESSDTQ GPKVTYIPPP

    PPEDEDSIFA HYQTGINFDK YDTILVEVSG HDAPPAILTF EEANLCQTLN

    NNIAKAGYTK LTPVQKYSIP IILAGRDLMA CAQTGSGKTA AFLLPILAHM

    MHDGITASRF KELQEPECII VAPTRELVNQ IYLEARKFSF GTCVRAVVIY

    GGTQLGHSIR QIVQGCNILC ATPGRLMDII GKEKIGLKQI KYLVLDEADR

    MLDMGFGPEM KKLISCPGMP SKEQRQTLMF SATFPEEIQR LAAEFLKSNY

    LFVAVGQVGG ACRDVQQTVL QVGQFSKREK LVEILRNIGD ERTMVFVETK

    KKADFIATFL CQEKISTTSI HGDREQRERE QALGDFRFGK CPVLVATSVA

    ARGLDIENVQ HVINFDLPST IDEYVHRIGR TGRCGNTGRA ISFFDLESDN

    HLAQPLVKVL TDAQQDVPAW LEEIAFSTYI PGFSGSTRGN VFASVDTRKG

    KSTLNTAGFS SSQAPNPVDD ESWD

  • UCSC uc003jqg.5; human. [Q9NQI0-1];


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