Human DDX11 ELISA Kit

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  • Alternative name

    Human CHL1-related protein 1 ELISA Kit;Human hCHLR1 ELISA Kit;Human DEAD/H-box protein 11 ELISA Kit;Human keratinocyte growth factor-regulated gene 2 protein ELISA Kit;Human KRG-2 ELISA Kit;Human CHL1 ELISA Kit;Human CHLR1 ELISA Kit;Human KRG2 ELISA Kit;Human WABS ELISA Kit;Human DEAD/H-box helicase 11 ELISA Kit;Human ATP-dependent DNA helicase DDX11 ELISA Kit;Human CHL1-like helicase homolog ELISA Kit;Human CHL1-related helicase gene-1 ELISA Kit;Human DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 ELISA Kit;Human DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S. cerevisiae) ELISA Kit;Human DEAD/H box protein 11 ELISA Kit;Human probable ATP-dependent DNA helicase DDX11 ELISA Kit;Human probable ATP-dependent RNA helicase DDX11 ELISA Kit;

  • Catalog
    E030688
  • species
    Human
  • GeneDDX11
  • Standard CurveHuman DDX11 ELISA Kit
  • Other Species Mouse Ddx11 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of DDX11. No significant cross-reactivity or interference between DDX11 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DDX11 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman DDX11 ELISA Kit allows for the in vitro quantitative determination of DDX11 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Intended Uses: This DDX11 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DDX11. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||DDX11 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-DDX11 antibody and an DDX11-HRP conjugate. The assay sample and buffer are incubated together with DDX11-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DDX11 concentration since DDX11 from samples and DDX11-HRP conjugate compete for the anti-DDX11 antibody binding site. Since the number of sites is limited, as more sites are occupied by DDX11 from the sample, fewer sites are left to bind DDX11-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDX11 concentration in each sample is interpolated from this standard curve.
  • Human ATP-dependent DNA helicase DDX11 Protein information
  • Uniprot ID DDX11_HUMAN
  • Uniprot AC Q96FC9; Q13333; Q86VQ4; Q86W62; Q92498; Q92770; Q92998; Q92999;
  • UniGene Hs.443960;
  • GeneID 1663
  • KEGG hsa:1663;
  • Human ATP-dependent DNA helicase DDX11 Protein SEQUENCE
  • SEQUENCE 970 AA; 108313 MW; 5BF49FE74E912B48 CRC64;

    MANETQKVGA IHFPFPFTPY SIQEDFMAEL YRVLEAGKIG IFESPTGTGK

    SLSLICGALS WLRDFEQKKR EEEARLLETG TGPLHDEKDE SLCLSSSCEG

    AAGTPRPAGE PAWVTQFVQK KEERDLVDRL KAEQARRKQR EERLQQLQHR

    VQLKYAAKRL RQEEEERENL LRLSREMLET GPEAERLEQL ESGEEELVLA

    EYESDEEKKV ASRVDEDEDD LEEEHITKIY YCSRTHSQLA QFVHEVKKSP

    FGKDVRLVSL GSRQNLCVNE DVKSLGSVQL INDRCVDMQR SRHEKKKGAE

    EEKPKRRRQE KQAACPFYNH EQMGLLRDEA LAEVKDMEQL LALGKEARAC

    PYYGSRLAIP AAQLVVLPYQ MLLHAATRQA AGIRLQDQVV IIDEAHNLID

    TITGMHSVEV SGSQLCQAHS QLLQYVERYG KRLKAKNLMY LKQILYLLEK

    FVAVLGGNIK QNPNTQSLSQ TGTELKTIND FLFQSQIDNI NLFKVQRYCE

    KSMISRKLFG FTERYGAVFS SREQPKLAGF QQFLQSLQPR TTEALAAPAD

    ESQASTLRPA SPLMHIQGFL AALTTANQDG RVILSRQGSL SQSTLKFLLL

    NPAVHFAQVV KECRAVVIAG GTMQPVSDFR QQLLACAGVE AERVVEFSCG

    HVIPPDNILP LVICSGISNQ PLEFTFQKRE LPQMMDEVGR ILCNLCGVVP

    GGVVCFFPSY EYLRQVHAHW EKGGLLGRLA ARKKIFQEPK SAHQVEQVLL

    AYSRCIQACG QERGQVTGAL LLSVVGGKMS EGINFSDNLG RCVVMVGMPF

    PNIRSAELQE KMAYLDQTLS PRPGTPREGS GGEPVHEGRQ PVHRQGHQAP

    EGFCQRSAPG PAICPAPCPG QAAGLDPSPC GGQSYLWPRH CCCAEVSPGE

    VGLFLMGNHT TAWRRALPLS CPLETVFVVG VVCGDPVTKV KPRRRVWSPE

    CCQDPGTGVS SRRRKWGNPE

  • UCSC uc001rjr.2; human. [Q96FC9-1];



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