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Catalog
E003065
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species
Human
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GeneASuA
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Standard Curve
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SpecificityThis assay recognizes recombinant and natural human Anti-Survivin antibody. No significant cross-reactivity or interference was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of human Anti-Survivin antibody is typically less than 0.78 U/mL.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero.
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Intended UseHuman ASuA ELISA Kit allows for the in vitro quantitative determination of ASuA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen specific to Anti-Survivin antibody. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antigen preparation specific for Anti-Survivin antibody and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Anti-Survivin antibody, biotin-conjugated antigen and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Anti-Survivin antibody in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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