Human IGFIIE ELISA Kit

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  • Catalog
    E030648
  • species
    Human
  • GeneIGFIIE
  • Standard CurveHuman IGFIIE ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Pro-Insulin Like Growth Factor II E-Peptide (IGFIIE). No significant cross-reactivity or interference between Pro-Insulin Like Growth Factor II E-Peptide (IGFIIE) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 15.2pg/mL.
  • Intended UseHuman IGFIIE ELISA Kit allows for the in vitro quantitative determination of IGFIIE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Pro-Insulin Like Growth Factor II E-Peptide (IGFIIE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Pro-Insulin Like Growth Factor II E-Peptide (IGFIIE) and unlabeled Pro-Insulin Like Growth Factor II E-Peptide (IGFIIE) (Standards or samples) with the pre-coated antibody specific to Pro-Insulin Like Growth Factor II E-Peptide (IGFIIE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Pro-Insulin Like Growth Factor II E-Peptide (IGFIIE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Pro-Insulin Like Growth Factor II E-Peptide (IGFIIE) in the sample.



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