SensitivityThe minimum detectable dose of human pro-IL1B is typically less than 3.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
Intended UseHuman pro-IL1B ELISA Kit allows for the in vitro quantitative determination of pro-IL1B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for pro-IL1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any pro-IL1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for pro-IL1B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of pro-IL1B bound in the initial step. The color development is stopped and the intensity of the color is measured.