Intended UseHuman PRND ELISA Kit allows for the in vitro quantitative determination of PRND , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PRND antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PRND antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PRND amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PRND can be calculated.
Human Prion-like protein doppel Protein information
Uniprot ID
PRND_HUMAN
Uniprot AC
Q9UKY0; A7U7M5; Q9H311; Q9H312; Q9NTM4;