Human PRNP ELISA Kit

Alternative name
Human PrP ELISA Kit;Human ASCR ELISA Kit;Human PrP27-30 ELISA Kit;Human PrP33-35C ELISA Kit;Human AltPrP ELISA Kit;Human PRIP ELISA Kit;Human CD230 ELISA Kit;Human CJD ELISA Kit;Human GSS ELISA Kit;Human KURU ELISA Kit;Human PrPc ELISA Kit;Human p27-30 ELISA Kit;Human prion protein ELISA Kit;Human major prion protein ELISA Kit;Human alternative prion protein ELISA Kit;Human CD230 antigen ELISA Kit;Human prion-related protein ELISA Kit;
Catalog
E030618
species
Human
GenePRNP
SpecificityThis assay has high sensitivity and excellent specificity for detection of human PRNP. No significant cross-reactivity or interference between human PRNP and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human PRNP is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman PRNP ELISA Kit allows for the in vitro quantitative determination of PRNP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRNP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRNP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRNP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRNP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Major prion protein Protein information
Uniprot ID PRIO_HUMAN
Uniprot AC Q96E70; Q9UP19;
UniGene Hs.472010; Hs.610285; Hs.721670;
GeneID 5621
KEGG hsa:5621;
Human Major prion protein Protein SEQUENCE
SEQUENCE 253 AA; 27661 MW; 43DB596BAAA66484 CRC64;
MANLGCWMLV LFVATWSDLG LCKKRPKPGG WNTGGSRYPG QGSPGGNRYP
PQGGGGWGQP HGGGWGQPHG GGWGQPHGGG WGQPHGGGWG QGGGTHSQWN
KPSKPKTNMK HMAGAAAAGA VVGGLGGYML GSAMSRPIIH FGSDYEDRYY
RENMHRYPNQ VYYRPMDEYS NQNNFVHDCV NITIKQHTVT TTTKGENFTE
TDVKMMERVV EQMCITQYER ESQAYYQRGS SMVLFSSPPV ILLISFLIFL
IVG

Human PRNP ELISA Kit

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