Human PRNP ELISA Kit

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  • Alternative name

    Human PrP ELISA Kit;Human ASCR ELISA Kit;Human PrP27-30 ELISA Kit;Human PrP33-35C ELISA Kit;Human AltPrP ELISA Kit;Human PRIP ELISA Kit;Human CD230 ELISA Kit;Human CJD ELISA Kit;Human GSS ELISA Kit;Human KURU ELISA Kit;Human PrPc ELISA Kit;Human p27-30 ELISA Kit;Human prion protein ELISA Kit;Human major prion protein ELISA Kit;Human alternative prion protein ELISA Kit;Human CD230 antigen ELISA Kit;Human prion-related protein ELISA Kit;

  • Catalog
    E030618
  • species
    Human
  • GenePRNP
  • Other Species Mouse Prnp ELISA KitBovine PRNP ELISA KitCanine PRNP ELISA KitGoat PRNP ELISA KitChicken PRNP ELISA KitHamster PRNP ELISA KitMonkey PRNP ELISA KitPorcine PRNP ELISA KitRabbit PRNP ELISA KitRat Prnp ELISA KitSheep PRNP ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human PRNP. No significant cross-reactivity or interference between human PRNP and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human PRNP is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
  • Intended UseHuman PRNP ELISA Kit allows for the in vitro quantitative determination of PRNP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRNP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRNP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRNP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRNP bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Major prion protein Protein information
  • Uniprot ID PRIO_HUMAN
  • Uniprot AC Q96E70; Q9UP19;
  • UniGene Hs.472010; Hs.610285; Hs.721670;
  • GeneID 5621
  • KEGG hsa:5621;
  • Human Major prion protein Protein SEQUENCE
  • SEQUENCE 253 AA; 27661 MW; 43DB596BAAA66484 CRC64;

    MANLGCWMLV LFVATWSDLG LCKKRPKPGG WNTGGSRYPG QGSPGGNRYP

    PQGGGGWGQP HGGGWGQPHG GGWGQPHGGG WGQPHGGGWG QGGGTHSQWN

    KPSKPKTNMK HMAGAAAAGA VVGGLGGYML GSAMSRPIIH FGSDYEDRYY

    RENMHRYPNQ VYYRPMDEYS NQNNFVHDCV NITIKQHTVT TTTKGENFTE

    TDVKMMERVV EQMCITQYER ESQAYYQRGS SMVLFSSPPV ILLISFLIFL

    IVG



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