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Human SP1 ELISA Kit

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  • Alternative name

    Human TSFP1 ELISA Kit;Human Sp1 transcription factor ELISA Kit;Human transcription factor Sp1 ELISA Kit;Human specificity protein 1 ELISA Kit;
  • Catalog
    E030522
  • species
    Human
  • GeneSP1
  • Standard CurveHuman SP1 ELISA Kit
  • Other Species Human SP1 / PSbeta1-G ELISA KitHuman SP1/PSbeta1-G ELISA KitHuman PSG1/SP1 ELISA KitMouse Sp1 ELISA KitMouse SP1 / PSbeta1-G ELISA KitRat Sp1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of SP1. No significant cross-reactivity or interference between SP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SP1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman SP1 ELISA Kit allows for the in vitro quantitative determination of SP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: SP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SP1 antibody and an SP1-HRP conjugate. The assay sample and buffer are incubated together with SP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SP1 concentration since SP1 from samples and SP1-HRP conjugate compete for the anti-SP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SP1 from the sample, fewer sites are left to bind SP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SP1 concentration in each sample is interpolated from this standard curve.
  • Human Transcription factor Sp1 Protein information
  • Uniprot ID SP1_HUMAN
  • Uniprot AC P08047; E4Z9M7; G5E9M8; Q86TN8; Q9H3Q5; Q9NR51; Q9NY21; Q9NYE7;
  • UniGene Hs.620754; Hs.649191;
  • GeneID 6667
  • KEGG hsa:6667;
  • Human Transcription factor Sp1 Protein SEQUENCE

  • SEQUENCE 785 AA; 80693 MW; 43893DBF6518B9EA CRC64;

    MSDQDHSMDE MTAVVKIEKG VGGNNGGNGN GGGAFSQARS SSTGSSSSTG

    GGGQESQPSP LALLAATCSR IESPNENSNN SQGPSQSGGT GELDLTATQL

    SQGANGWQII SSSSGATPTS KEQSGSSTNG SNGSESSKNR TVSGGQYVVA

    AAPNLQNQQV LTGLPGVMPN IQYQVIPQFQ TVDGQQLQFA ATGAQVQQDG

    SGQIQIIPGA NQQIITNRGS GGNIIAAMPN LLQQAVPLQG LANNVLSGQT

    QYVTNVPVAL NGNITLLPVN SVSAATLTPS SQAVTISSSG SQESGSQPVT

    SGTTISSASL VSSQASSSSF FTNANSYSTT TTTSNMGIMN FTTSGSSGTN

    SQGQTPQRVS GLQGSDALNI QQNQTSGGSL QAGQQKEGEQ NQQTQQQQIL

    IQPQLVQGGQ ALQALQAAPL SGQTFTTQAI SQETLQNLQL QAVPNSGPII

    IRTPTVGPNG QVSWQTLQLQ NLQVQNPQAQ TITLAPMQGV SLGQTSSSNT

    TLTPIASAAS IPAGTVTVNA AQLSSMPGLQ TINLSALGTS GIQVHPIQGL

    PLAIANAPGD HGAQLGLHGA GGDGIHDDTA GGEEGENSPD AQPQAGRRTR

    REACTCPYCK DSEGRGSGDP GKKKQHICHI QGCGKVYGKT SHLRAHLRWH

    TGERPFMCTW SYCGKRFTRS DELQRHKRTH TGEKKFACPE CPKRFMRSDH

    LSKHIKTHQN KKGGPGVALS VGTLPLDSGA GSEGSGTATP SALITTNMVA

    MEAICPEGIA RLANSGINVM QVADLQSINI SGNGF

  • UCSC uc001scw.4; human. [P08047-1];


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