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Human PAPPA ELISA Kit

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  • Alternative name

    Human Insulin-like growth factor-dependent IGF-binding protein 4 protease ELISA Kit;Human IGF-dependent IGFBP-4 protease ELISA Kit;Human IGFBP-4ase ELISA Kit;Human Pregnancy-associated plasma protein A ELISA Kit;Human PAPP-A ELISA Kit;Human ASBABP2 ELISA Kit;Human DIPLA1 ELISA Kit;Human PAPA ELISA Kit;Human PAPPA1 ELISA Kit;Human pappalysin 1 ELISA Kit;Human pappalysin-1 ELISA Kit;Human aspecific BCL2 ARE-binding protein 2 ELISA Kit;Human differentially placenta 1 expressed protein ELISA Kit;Human insulin-like growth factor-dependent IGF binding protein-4 protease ELISA Kit;Human pregnacy-associated plasma protein A ELISA Kit;Human pregnancy-associated plasma protein A, pappalysin 1 ELISA Kit;
  • Catalog
    E030511
  • species
    Human
  • GenePAPPA
  • Standard CurveHuman PAPPA ELISA Kit
  • Other Species Mouse Pappa ELISA KitHuman PAPPA-AS1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Pregnancy Associated Plasma Protein A (PAPPA). No significant cross-reactivity or interference between Pregnancy Associated Plasma Protein A (PAPPA) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 13.5pg/mL.
  • Intended UseHuman PAPPA ELISA Kit allows for the in vitro quantitative determination of PAPPA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pregnancy Associated Plasma Protein A (PAPPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pregnancy Associated Plasma Protein A (PAPPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pregnancy Associated Plasma Protein A (PAPPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pregnancy Associated Plasma Protein A (PAPPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Pappalysin-1 Protein information
  • Uniprot ID PAPP1_HUMAN
  • Uniprot AC Q13219; B1AMF9; Q08371; Q68G52; Q9UDK7;
  • UniGene Hs.643599;
  • GeneID 5069
  • KEGG hsa:5069;
  • Human Pappalysin-1 Protein SEQUENCE

  • SEQUENCE 1627 AA; 180973 MW; 202ECA62C1107207 CRC64;

    MRLWSWVLHL GLLSAALGCG LAERPRRARR DPRAGRPPRP AAGPATCATR

    AARGRRASPP PPPPPGGAWE AVRVPRRRQQ REARGATEEP SPPSRALYFS

    GRGEQLRLRA DLELPRDAFT LQVWLRAEGG QRSPAVITGL YDKCSYISRD

    RGWVVGIHTI SDQDNKDPRY FFSLKTDRAR QVTTINAHRS YLPGQWVYLA

    ATYDGQFMKL YVNGAQVATS GEQVGGIFSP LTQKCKVLML GGSALNHNYR

    GYIEHFSLWK VARTQREILS DMETHGAHTA LPQLLLQENW DNVKHAWSPM

    KDGSSPKVEF SNAHGFLLDT SLEPPLCGQT LCDNTEVIAS YNQLSSFRQP

    KVVRYRVVNL YEDDHKNPTV TREQVDFQHH QLAEAFKQYN ISWELDVLEV

    SNSSLRRRLI LANCDISKIG DENCDPECNH TLTGHDGGDC RHLRHPAFVK

    KQHNGVCDMD CNYERFNFDG GECCDPEITN VTQTCFDPDS PHRAYLDVNE

    LKNILKLDGS THLNIFFAKS SEEELAGVAT WPWDKEALMH LGGIVLNPSF

    YGMPGHTHTM IHEIGHSLGL YHVFRGISEI QSCSDPCMET EPSFETGDLC

    NDTNPAPKHK SCGDPGPGND TCGFHSFFNT PYNNFMSYAD DDCTDSFTPN

    QVARMHCYLD LVYQGWQPSR KPAPVALAPQ VLGHTTDSVT LEWFPPIDGH

    FFERELGSAC HLCLEGRILV QYASNASSPM PCSPSGHWSP REAEGHPDVE

    QPCKSSVRTW SPNSAVNPHT VPPACPEPQG CYLELEFLYP LVPESLTIWV

    TFVSTDWDSS GAVNDIKLLA VSGKNISLGP QNVFCDVPLT IRLWDVGEEV

    YGIQIYTLDE HLEIDAAMLT STADTPLCLQ CKPLKYKVVR DPPLQMDVAS

    ILHLNRKFVD MDLNLGSVYQ YWVITISGTE ESEPSPAVTY IHGSGYCGDG

    IIQKDQGEQC DDMNKINGDG CSLFCRQEVS FNCIDEPSRC YFHDGDGVCE

    EFEQKTSIKD CGVYTPQGFL DQWASNASVS HQDQQCPGWV IIGQPAASQV

    CRTKVIDLSE GISQHAWYPC TISYPYSQLA QTTFWLRAYF SQPMVAAAVI

    VHLVTDGTYY GDQKQETISV QLLDTKDQSH DLGLHVLSCR NNPLIIPVVH

    DLSQPFYHSQ AVRVSFSSPL VAISGVALRS FDNFDPVTLS SCQRGETYSP

    AEQSCVHFAC EKTDCPELAV ENASLNCSSS DRYHGAQCTV SCRTGYVLQI

    RRDDELIKSQ TGPSVTVTCT EGKWNKQVAC EPVDCSIPDH HQVYAASFSC

    PEGTTFGSQC SFQCRHPAQL KGNNSLLTCM EDGLWSFPEA LCELMCLAPP

    PVPNADLQTA RCRENKHKVG SFCKYKCKPG YHVPGSSRKS KKRAFKTQCT

    QDGSWQEGAC VPVTCDPPPP KFHGLYQCTN GFQFNSECRI KCEDSDASQG

    LGSNVIHCRK DGTWNGSFHV CQEMQGQCSV PNELNSNLKL QCPDGYAIGS

    ECATSCLDHN SESIILPMNV TVRDIPHWLN PTRVERVVCT AGLKWYPHPA

    LIHCVKGCEP FMGDNYCDAI NNRAFCNYDG GDCCTSTVKT KKVTPFPMSC

    DLQGDCACRD PQAQEHSRKD LRGYSHG

  • UCSC uc004bjn.4; human.;


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