Human PFDN1 ELISA Kit

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  • Alternative name

    Human PFD1 ELISA Kit;Human PDF ELISA Kit;Human prefoldin subunit 1 ELISA Kit;Human prefoldin 1 ELISA Kit;

  • Catalog
    E030490
  • species
    Human
  • GenePFDN1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Prefoldin Subunit 1 (PFDN1). No significant cross-reactivity or interference between Prefoldin Subunit 1 (PFDN1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.112ng/mL.
  • Intended UseHuman PFDN1 ELISA Kit allows for the in vitro quantitative determination of PFDN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prefoldin Subunit 1 (PFDN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prefoldin Subunit 1 (PFDN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prefoldin Subunit 1 (PFDN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prefoldin Subunit 1 (PFDN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Prefoldin subunit 1 Protein information
  • Uniprot ID PFD1_HUMAN
  • Uniprot AC O60925; B2RD02; Q53F95; Q96EX6;
  • UniGene Hs.483564;
  • GeneID 5201
  • KEGG hsa:5201;
  • Human Prefoldin subunit 1 Protein SEQUENCE
  • SEQUENCE 122 AA; 14210 MW; 4C88216612864F87 CRC64;

    MAAPVDLELK KAFTELQAKV IDTQQKVKLA DIQIEQLNRT KKHAHLTDTE

    IMTLVDETNM YEGVGRMFIL QSKEAIHSQL LEKQKIAEEK IKELEQKKSY

    LERSVKEAED NIREMLMARR AQ

  • UCSC uc003lff.2; human.;



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