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Alternative name
Human PFD1 ELISA Kit;Human PDF ELISA Kit;Human prefoldin subunit 1 ELISA Kit;Human prefoldin 1 ELISA Kit;
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Catalog
E030490
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species
Human
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GenePFDN1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Prefoldin Subunit 1 (PFDN1).
No significant cross-reactivity or interference between Prefoldin Subunit 1 (PFDN1) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.112ng/mL.
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Intended UseHuman PFDN1 ELISA Kit allows for the in vitro quantitative determination of PFDN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prefoldin Subunit 1 (PFDN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prefoldin Subunit 1 (PFDN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prefoldin Subunit 1 (PFDN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prefoldin Subunit 1 (PFDN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Human Prefoldin subunit 1 Protein information
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Uniprot ID
PFD1_HUMAN
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Uniprot AC
O60925; B2RD02; Q53F95; Q96EX6;
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UniGene
Hs.483564;
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GeneID
5201
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KEGG
hsa:5201;
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Human Prefoldin subunit 1 Protein SEQUENCE
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SEQUENCE 122 AA; 14210 MW; 4C88216612864F87 CRC64;
MAAPVDLELK KAFTELQAKV IDTQQKVKLA DIQIEQLNRT KKHAHLTDTE
IMTLVDETNM
YEGVGRMFIL QSKEAIHSQL LEKQKIAEEK IKELEQKKSY
LERSVKEAED NIREMLMARR
AQ
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UCSC
uc003lff.2; human.;
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