SensitivityThe minimum detectable dose of Human pPLA2 is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
Intended UseHuman pPLA2 ELISA Kit allows for the in vitro quantitative determination of pPLA2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with pPLA2. During the reaction, pPLA2 in the sample or standard competes with a fixed amount of pPLA2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to pPLA2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of pPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve.