Human TFPI2 ELISA Kit

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  • Alternative name

    Human TFPI-2 ELISA Kit;Human placental protein 5 ELISA Kit;Human PP5 ELISA Kit;Human REF1 ELISA Kit;Human tissue factor pathway inhibitor 2 ELISA Kit;Human retinal pigment epithelium cell factor 1 ELISA Kit;

  • Catalog
    E030376
  • species
    Human
  • GeneTFPI2
  • Other Species Human TFPI2 ELISA KitMouse Tfpi2 ELISA KitBovine TFPI2 ELISA Kit
  • SpecificityThe Human PP5 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human PP5 proteins within the range of 156 pg/ml - 10000 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity10 pg/ml.
  • Intended UseHuman TFPI2 ELISA Kit allows for the in vitro quantitative determination of TFPI2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||The Human PP5 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PP5 in Cell Culture Supernatants, Serum. This assay employs an antibody specific for Human PP5 coated on a 96-well plate. Standards and samples are pipetted into the wells and PP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: TFPI2, also called Tissue factor pathway inhibitor 2, is a protein that in humans is encoded by the TFPI2 gene. It is mapped to 7q21.3. This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Qualitative PCR and microarray analyses showed that expression of TFPI2 was inversely correlated with that of MIR616 in prostate cancer cell lines, primary tumors of various grades, and normal tissue.
  • Human Tissue factor pathway inhibitor 2 Protein information
  • Uniprot ID TFPI2_HUMAN
  • Uniprot AC P48307; Q66ME8; Q8NAK6; Q9UC86;
  • UniGene Hs.438231;
  • GeneID 7980
  • KEGG hsa:7980;
  • Human Tissue factor pathway inhibitor 2 Protein SEQUENCE
  • SEQUENCE 235 AA; 26934 MW; 975ABA5C53F7C65F CRC64;

    MDPARPLGLS ILLLFLTEAA LGDAAQEPTG NNAEICLLPL DYGPCRALLL

    RYYYDRYTQS CRQFLYGGCE GNANNFYTWE ACDDACWRIE KVPKVCRLQV

    SVDDQCEGST EKYFFNLSSM TCEKFFSGGC HRNRIENRFP DEATCMGFCA

    PKKIPSFCYS PKDEGLCSAN VTRYYFNPRY RTCDAFTYTG CGGNDNNFVS

    REDCKRACAK ALKKKKKMPK LRFASRIRKI RKKQF

  • UCSC uc003umy.2; human. [P48307-1];


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