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Human UBB ELISA Kit

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  • Alternative name

    Human HEL-S-50 ELISA Kit;Human ubiquitin B ELISA Kit;Human polyubiquitin-B ELISA Kit;Human epididymis secretory protein Li 50 ELISA Kit;Human polyubiquitin B ELISA Kit;
  • Catalog
    E030174
  • species
    Human
  • GeneUBB
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 3.75ng/ml
  • Intended UseHuman UBB ELISA Kit allows for the in vitro quantitative determination of UBB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human UBBantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human UBB antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human UBBamount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofhuman UBBcan be calculated.
  • Human Ubiquitin Protein information
  • Uniprot ID UBB_HUMAN
  • Uniprot AC Q9UPK7;
  • UniGene Hs.356190; Hs.730603; Hs.741549;
  • GeneID 7314
  • KEGG hsa:7314;
  • Human Ubiquitin Protein SEQUENCE

  • SEQUENCE 229 AA; 25762 MW; 33011162F1C48BB1 CRC64;

    MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL

    EDGRTLSDYN IQKESTLHLV LRLRGGMQIF VKTLTGKTIT LEVEPSDTIE

    NVKAKIQDKE GIPPDQQRLI FAGKQLEDGR TLSDYNIQKE STLHLVLRLR

    GGMQIFVKTL TGKTITLEVE PSDTIENVKA KIQDKEGIPP DQQRLIFAGK

    QLEDGRTLSD YNIQKESTLH LVLRLRGGC




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