Human POLB ELISA Kit

Alternative name
Human DNA polymerase beta ELISA Kit;Human DNA pol beta ELISA Kit;Human DNA polymerase beta subunit ELISA Kit;Human polymerase (DNA directed), beta ELISA Kit;Human polymerase (DNA) beta ELISA Kit;
Catalog
E030133
species
Human
GenePOLB
Standard Curve
Other Species Mouse Polb ELISA Kit Bovine POLB ELISA Kit Rat Polb ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Polymerase DNA Directed Beta (POLb). No significant cross-reactivity or interference between Polymerase DNA Directed Beta (POLb) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.051ng/mL.
Intended UseHuman POLB ELISA Kit allows for the in vitro quantitative determination of POLB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polymerase DNA Directed Beta (POLb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Polymerase DNA Directed Beta (POLb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Polymerase DNA Directed Beta (POLb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Polymerase DNA Directed Beta (POLb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human DNA polymerase beta Protein information
Uniprot ID DPOLB_HUMAN
Uniprot AC P06746; B2RC78; Q3KP48; Q6FI34;
UniGene Hs.654484; Hs.661106;
GeneID 5423
KEGG hsa:5423;
Human DNA polymerase beta Protein SEQUENCE
SEQUENCE 335 AA; 38178 MW; FF9A6D0E6F9A9487 CRC64;
MSKRKAPQET LNGGITDMLT ELANFEKNVS QAIHKYNAYR KAASVIAKYP
HKIKSGAEAK KLPGVGTKIA EKIDEFLATG KLRKLEKIRQ DDTSSSINFL
TRVSGIGPSA ARKFVDEGIK TLEDLRKNED KLNHHQRIGL KYFGDFEKRI
PREEMLQMQD IVLNEVKKVD SEYIATVCGS FRRGAESSGD MDVLLTHPSF
TSESTKQPKL LHQVVEQLQK VHFITDTLSK GETKFMGVCQ LPSKNDEKEY
PHRRIDIRLI PKDQYYCGVL YFTGSDIFNK NMRAHALEKG FTINEYTIRP
LGVTGVAGEP LPVDSEKDIF DYIQWKYREP KDRSE
UCSC uc003xoz.3; human.;

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Category

Human POLB ELISA Kit

Human DNA polymerase beta ELISA Kit;Human DNA pol beta ELISA Kit;Human DNA polymerase beta subunit ELISA Kit;Human polymerase (DNA directed), beta ELISA Kit;Human polymerase (DNA) beta ELISA Kit;