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Human POLR2A ELISA Kit

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  • Alternative name

    Human RNA polymerase II subunit B1 ELISA Kit;Human DNA-directed RNA polymerase II subunit A ELISA Kit;Human DNA-directed RNA polymerase III largest subunit ELISA Kit;Human RNA-directed RNA polymerase II subunit RPB1 ELISA Kit;Human POLR2 ELISA Kit;Human POLRA ELISA Kit;Human RPB1 ELISA Kit;Human RPBh1 ELISA Kit;Human RPO2 ELISA Kit;Human RPOL2 ELISA Kit;Human RpIILS ELISA Kit;Human hRPB220 ELISA Kit;Human hsRPB1 ELISA Kit;Human RNA polymerase II subunit A ELISA Kit;Human DNA-directed RNA polymerase II subunit RPB1 ELISA Kit;Human DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit ELISA Kit;Human polymerase (RNA) II (DNA directed) polypeptide A, 220kDa ELISA Kit;Human polymerase (RNA) II subunit A ELISA Kit;
  • Catalog
    E030113
  • species
    Human
  • GenePOLR2A
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human POLR2A. No significant cross-reactivity or interference between human POLR2A and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human POLR2A is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
  • Intended UseHuman POLR2A ELISA Kit allows for the in vitro quantitative determination of POLR2A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for POLR2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any POLR2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLR2A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLR2A bound in the initial step.
  • Human DNA-directed RNA polymerase II subunit RPB1 Protein information
  • Uniprot ID RPB1_HUMAN
  • Uniprot AC P24928; A6NN93; B9EH88; Q6NX41;
  • UniGene Hs.270017;
  • GeneID 5430
  • KEGG hsa:5430;
  • Human DNA-directed RNA polymerase II subunit RPB1 Protein SEQUENCE

  • SEQUENCE 1970 AA; 217176 MW; 28D6FD25693A6472 CRC64;

    MHGGGPPSGD SACPLRTIKR VQFGVLSPDE LKRMSVTEGG IKYPETTEGG

    RPKLGGLMDP RQGVIERTGR CQTCAGNMTE CPGHFGHIEL AKPVFHVGFL

    VKTMKVLRCV CFFCSKLLVD SNNPKIKDIL AKSKGQPKKR LTHVYDLCKG

    KNICEGGEEM DNKFGVEQPE GDEDLTKEKG HGGCGRYQPR IRRSGLELYA

    EWKHVNEDSQ EKKILLSPER VHEIFKRISD EECFVLGMEP RYARPEWMIV

    TVLPVPPLSV RPAVVMQGSA RNQDDLTHKL ADIVKINNQL RRNEQNGAAA

    HVIAEDVKLL QFHVATMVDN ELPGLPRAMQ KSGRPLKSLK QRLKGKEGRV

    RGNLMGKRVD FSARTVITPD PNLSIDQVGV PRSIAANMTF AEIVTPFNID

    RLQELVRRGN SQYPGAKYII RDNGDRIDLR FHPKPSDLHL QTGYKVERHM

    CDGDIVIFNR QPTLHKMSMM GHRVRILPWS TFRLNLSVTT PYNADFDGDE

    MNLHLPQSLE TRAEIQELAM VPRMIVTPQS NRPVMGIVQD TLTAVRKFTK

    RDVFLERGEV MNLLMFLSTW DGKVPQPAIL KPRPLWTGKQ IFSLIIPGHI

    NCIRTHSTHP DDEDSGPYKH ISPGDTKVVV ENGELIMGIL CKKSLGTSAG

    SLVHISYLEM GHDITRLFYS NIQTVINNWL LIEGHTIGIG DSIADSKTYQ

    DIQNTIKKAK QDVIEVIEKA HNNELEPTPG NTLRQTFENQ VNRILNDARD

    KTGSSAQKSL SEYNNFKSMV VSGAKGSKIN ISQVIAVVGQ QNVEGKRIPF

    GFKHRTLPHF IKDDYGPESR GFVENSYLAG LTPTEFFFHA MGGREGLIDT

    AVKTAETGYI QRRLIKSMES VMVKYDATVR NSINQVVQLR YGEDGLAGES

    VEFQNLATLK PSNKAFEKKF RFDYTNERAL RRTLQEDLVK DVLSNAHIQN

    ELEREFERMR EDREVLRVIF PTGDSKVVLP CNLLRMIWNA QKIFHINPRL

    PSDLHPIKVV EGVKELSKKL VIVNGDDPLS RQAQENATLL FNIHLRSTLC

    SRRMAEEFRL SGEAFDWLLG EIESKFNQAI AHPGEMVGAL AAQSLGEPAT

    QMTLNTFHYA GVSAKNVTLG VPRLKELINI SKKPKTPSLT VFLLGQSARD

    AERAKDILCR LEHTTLRKVT ANTAIYYDPN PQSTVVAEDQ EWVNVYYEMP

    DFDVARISPW LLRVELDRKH MTDRKLTMEQ IAEKINAGFG DDLNCIFNDD

    NAEKLVLRIR IMNSDENKMQ EEEEVVDKMD DDVFLRCIES NMLTDMTLQG

    IEQISKVYMH LPQTDNKKKI IITEDGEFKA LQEWILETDG VSLMRVLSEK

    DVDPVRTTSN DIVEIFTVLG IEAVRKALER ELYHVISFDG SYVNYRHLAL

    LCDTMTCRGH LMAITRHGVN RQDTGPLMKC SFEETVDVLM EAAAHGESDP

    MKGVSENIML GQLAPAGTGC FDLLLDAEKC KYGMEIPTNI PGLGAAGPTG

    MFFGSAPSPM GGISPAMTPW NQGATPAYGA WSPSVGSGMT PGAAGFSPSA

    ASDASGFSPG YSPAWSPTPG SPGSPGPSSP YIPSPGGAMS PSYSPTSPAY

    EPRSPGGYTP QSPSYSPTSP SYSPTSPSYS PTSPNYSPTS PSYSPTSPSY

    SPTSPSYSPT SPSYSPTSPS YSPTSPSYSP TSPSYSPTSP SYSPTSPSYS

    PTSPSYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPS YSPTSPNYSP

    TSPNYTPTSP SYSPTSPSYS PTSPNYTPTS PNYSPTSPSY SPTSPSYSPT

    SPSYSPSSPR YTPQSPTYTP SSPSYSPSSP SYSPASPKYT PTSPSYSPSS

    PEYTPTSPKY SPTSPKYSPT SPKYSPTSPT YSPTTPKYSP TSPTYSPTSP

    VYTPTSPKYS PTSPTYSPTS PKYSPTSPTY SPTSPKGSTY SPTSPGYSPT

    SPTYSLTSPA ISPDDSDEEN

  • UCSC uc002ghe.4; human. [P24928-1];



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