Human PMFBP1 ELISA Kit

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  • Alternative name

    Human PMF-1-binding protein ELISA Kit;Human polyamine modulated factor 1 binding protein 1 ELISA Kit;Human polyamine-modulated factor 1-binding protein 1 ELISA Kit;Human PMF-1 binding protein ELISA Kit;

  • Catalog
    E030048
  • species
    Human
  • GenePMFBP1
  • Standard CurveHuman PMFBP1 ELISA Kit
  • Other Species Mouse Pmfbp1 ELISA KitRat Pmfbp1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Polyamine Modulated Factor 1 Binding Protein 1 (PMFBP1). No significant cross-reactivity or interference between Polyamine Modulated Factor 1 Binding Protein 1 (PMFBP1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.051ng/mL.
  • Intended UseHuman PMFBP1 ELISA Kit allows for the in vitro quantitative determination of PMFBP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polyamine Modulated Factor 1 Binding Protein 1 (PMFBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Polyamine Modulated Factor 1 Binding Protein 1 (PMFBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Polyamine Modulated Factor 1 Binding Protein 1 (PMFBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Polyamine Modulated Factor 1 Binding Protein 1 (PMFBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Polyamine-modulated factor 1-binding protein 1 Protein information
  • Uniprot ID PMFBP_HUMAN
  • Uniprot AC Q8TBY8; B3KVI9; H7BY07; Q8NA09; Q9BY16; Q9H0H4;
  • UniGene Hs.714939;
  • GeneID 83449
  • KEGG hsa:83449;
  • Human Polyamine-modulated factor 1-binding protein 1 Protein SEQUENCE
  • SEQUENCE 1022 AA; 119033 MW; FCC6161294E55205 CRC64;

    MKDEAGERDR EVSSLNSKLL SLQLDIKNLH DVCKRQRKTL QDNQLCMEEA

    MNSSHDKKQA QALAFEESEV EFGSSKQCHL RQLQQLKKKL LVLQQELEFH

    TEELQTSYYS LRQYQSILEK QTSDLVLLHH HCKLKEDEVI LYEEEMGNHN

    ENTGEKLHLA QEQLALAGDK IASLERSLNL YRDKYQSSLS NIELLECQVK

    MLQGELGGIM GQEPENKGDH SKVRIYTSPC MIQEHQETQK RLSEVWQKVS

    QQDDLIQELR NKLACSNALV LEREKALIKL QADFASCTAT HRYPPSSSEE

    CEDIKKILKH LQEQKDSQCL HVEEYQNLVK DLRVELEAVS EQKRNIMKDM

    MKLELDLHGL REETSAHIER KDKDITILQC RLQELQLEFT ETQKLTLKKD

    KFLQEKDEML QELEKKLTQV QNSLLKKEKE LEKQQCMATE LEMTVKEAKQ

    DKSKEAECKA LQAEVQKLKN SLEEAKQQER LAGEAPAAQQ AAQCKEEAAL

    AGCHLEDTQR KLQKGLLLDK QKADTIQELQ RELQMLQKES SMAEKEQTSN

    RKRVEELSLE LSEALRKLEN SDKEKRQLQK TVAEQDMKMN DMLDRIKHQH

    REQGSIKCKL EEDLQEATKL LEDKREQLKK SKEHEKLMEG ELEALRQEFK

    KKDKTLKENS RKLEEENENL RAELQCCSTQ LESSLNKYNT SQQVIQDLNK

    EIALQKESLM SLQAQLDKAL QKEKHYLQTT ITKEAYDALS RKSAACQDDL

    TQALEKLNHV TSETKSLQQS LTQTQEKKAQ LEEEIIAYEE RMKKLNTELR

    KLRGFHQESE LEVHAFDKKL EEMSCQVLQW QKQHQNDLKM LAAKEEQLRE

    FQEEMAALKE NLLEDDKEPC CLPQWSVPKD TCRLYRGNDQ IMTNLEQWAK

    QQKVANEKLG NQLREQVKYI AKLSGEKDHL HSVMVHLQQE NKKLKKEIEE

    KKMKAENTRL CTKALGPSRT ESTQREKVCG TLGWKGLPQD MGQRMDLTKY

    IGMPHCPGTS AIGQKNKCDF FL

  • UCSC uc002fcd.4; human. [Q8TBY8-1];


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