Human MTPAP ELISA Kit

Alternative name
Human PAP ELISA Kit;Human PAP-associated domain-containing protein 1 ELISA Kit;Human polynucleotide adenylyltransferase ELISA Kit;Human terminal uridylyltransferase 1 ELISA Kit;Human TUTase 1 ELISA Kit;Human mtPAP ELISA Kit;Human PAPD1 ELISA Kit;Human SPAX4 ELISA Kit;Human mitochondrial poly(A) polymerase ELISA Kit;Human poly(A) RNA polymerase, mitochondrial ELISA Kit;
Catalog
E030036
species
Human
GeneMTPAP
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188ng/ml
Intended UseHuman MTPAP ELISA Kit allows for the in vitro quantitative determination of MTPAP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MTPAP. During the reaction,Human MTPAP in the sample or standard competes with a fixed amount of biotin-labeled Human MTPAP for sites on a pre-coated Monoclonal antibody specific to Human MTPAP. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human MTPAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Poly(A) RNA polymerase, mitochondrial Protein information
Uniprot ID PAPD1_HUMAN
Uniprot AC Q9NVV4; D3DRX0; Q659E3; Q6P7E5; Q9HA74;
UniGene Hs.173946;
GeneID 55149
KEGG hsa:55149;
Human Poly(A) RNA polymerase, mitochondrial Protein SEQUENCE
SEQUENCE 582 AA; 66172 MW; EBA5BECEA39A5090 CRC64;
MAVPGVGLLT RLNLCARRRT RVQRPIVRLL SCPGTVAKDL RRDEQPSGSV
ETGFEDKIPK RRFSEMQNER REQAQRTVLI HCPEKISENK FLKYLSQFGP
INNHFFYESF GLYAVVEFCQ KESIGSLQNG THTPSTAMET AIPFRSRFFN
LKLKNQTSER SRVRSSNQLP RSNKQLFELL CYAESIDDQL NTLLKEFQLT
EENTKLRYLT CSLIEDMAAA YFPDCIVRPF GSSVNTFGKL GCDLDMFLDL
DETRNLSAHK ISGNFLMEFQ VKNVPSERIA TQKILSVLGE CLDHFGPGCV
GVQKILNARC PLVRFSHQAS GFQCDLTTNN RIALTSSELL YIYGALDSRV
RALVFSVRCW ARAHSLTSSI PGAWITNFSL TMMVIFFLQR RSPPILPTLD
SLKTLADAED KCVIEGNNCT FVRDLSRIKP SQNTETLELL LKEFFEYFGN
FAFDKNSINI RQGREQNKPD SSPLYIQNPF ETSLNISKNV SQSQLQKFVD
LARESAWILQ QEDTDRPSIS SNRPWGLVSL LLPSAPNRKS FTKKKSNKFA
IETVKNLLES LKGNRTENFT KTSGKRTIST QT
UCSC uc001iva.5; human. [Q9NVV4-1];

Category

Human MTPAP ELISA Kit