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Human PARP15 ELISA Kit

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  • Alternative name

    Human PARP-15 ELISA Kit;Human ADP-ribosyltransferase diphtheria toxin-like 7 ELISA Kit;Human ARTD7 ELISA Kit;Human B-aggressive lymphoma protein 3 ELISA Kit;Human BAL3 ELISA Kit;Human pART7 ELISA Kit;Human poly(ADP-ribose) polymerase family member 15 ELISA Kit;Human poly [ADP-ribose] polymerase 15 ELISA Kit;Human B-aggressive lymphoma 3 ELISA Kit;
  • Catalog
    E030025
  • species
    Human
  • GenePARP15
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PARP15. No significant cross-reactivity or interference between Human PARP15 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe limit of detection of Human PARP15 defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 0.1 ng/mL (mean of 6 independent assays).
  • Intended UseHuman PARP15 ELISA Kit allows for the in vitro quantitative determination of PARP15 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs a two-site sandwich ELISA to quantitative PARP15 in Human serum, plasma. An antibody specific for PARP15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PARP15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for PARP15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PARP15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Poly [ADP-ribose] polymerase 15 Protein information
  • Uniprot ID PAR15_HUMAN
  • Uniprot AC Q460N3; J3KR47; Q8N1K3;
  • UniGene Hs.120250;
  • GeneID 165631
  • KEGG hsa:165631;
  • Human Poly [ADP-ribose] polymerase 15 Protein SEQUENCE

  • SEQUENCE 678 AA; 74576 MW; E299386DA4B2BF8C CRC64;

    MAAPGPLPAA ALSPGAPTPR ELMHGVAGVT SRAGRDREAG SVLPAGNRGA

    RKASRRSSSR SMSRDNKFSK KDCLSIRNVV ASIQTKEGLN LKLISGDVLY

    IWADVIVNSV PMNLQLGGGP LSRAFLQKAG PMLQKELDDR RRETEEKVGN

    IFMTSGCNLD CKAVLHAVAP YWNNGAETSW QIMANIIKKC LTTVEVLSFS

    SITFPMIGTG SLQFPKAVFA KLILSEVFEY SSSTRPITSP LQEVHFLVYT

    NDDEGCQAFL DEFTNWSRIN PNKARIPMAG DTQGVVGTVS KPCFTAYEMK

    IGAITFQVAT GDIATEQVDV IVNSTARTFN RKSGVSRAIL EGAGQAVESE

    CAVLAAQPHR DFIITPGGCL KCKIIIHVPG GKDVRKTVTS VLEECEQRKY

    TSVSLPAIGT GNAGKNPITV ADNIIDAIVD FSSQHSTPSL KTVKVVIFQP

    ELLNIFYDSM KKRDLSASLN FQSTFSMTTC NLPEHWTDMN HQLFCMVQLE

    PGQSEYNTIK DKFTRTCSSY AIEKIERIQN AFLWQSYQVK KRQMDIKNDH

    KNNERLLFHG TDADSVPYVN QHGFNRSCAG KNAVSYGKGT YFAVDASYSA

    KDTYSKPDSN GRKHMYVVRV LTGVFTKGRA GLVTPPPKNP HNPTDLFDSV

    TNNTRSPKLF VVFFDNQAYP EYLITFTA

  • UCSC uc003efm.3; human. [Q460N3-1];


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