Human PLK1 ELISA Kit

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  • Alternative name

    Human Polo-like kinase 1 ELISA Kit;Human PLK-1 ELISA Kit;Human serine/threonine-protein kinase 13 ELISA Kit;Human STPK13 ELISA Kit;Human PLK ELISA Kit;Human polo like kinase 1 ELISA Kit;Human serine/threonine-protein kinase PLK1 ELISA Kit;Human cell cycle regulated protein kinase ELISA Kit;Human polo (Drosophia)-like kinase ELISA Kit;

  • Catalog
    E029987
  • species
    Human
  • GenePLK1
  • Standard CurveHuman PLK1 ELISA Kit
  • Other Species Human P-PLK1 ELISA KitMouse P-PLK1 ELISA KitMouse Plk1 ELISA KitBovine PLK1 ELISA KitRat Plk1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human PLK1. No significant cross-reactivity or interference between human PLK1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human PLK1 is typically less than 9.77 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman PLK1 ELISA Kit allows for the in vitro quantitative determination of PLK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLK1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Serine/threonine-protein kinase PLK1 Protein information
  • Uniprot ID PLK1_HUMAN
  • Uniprot AC P53350; Q15153; Q99746;
  • UniGene Hs.592049;
  • GeneID 5347
  • KEGG hsa:5347;
  • Human Serine/threonine-protein kinase PLK1 Protein SEQUENCE
  • SEQUENCE 603 AA; 68255 MW; 178C2F13C10E8206 CRC64;

    MSAAVTAGKL ARAPADPGKA GVPGVAAPGA PAAAPPAKEI PEVLVDPRSR

    RRYVRGRFLG KGGFAKCFEI SDADTKEVFA GKIVPKSLLL KPHQREKMSM

    EISIHRSLAH QHVVGFHGFF EDNDFVFVVL ELCRRRSLLE LHKRRKALTE

    PEARYYLRQI VLGCQYLHRN RVIHRDLKLG NLFLNEDLEV KIGDFGLATK

    VEYDGERKKT LCGTPNYIAP EVLSKKGHSF EVDVWSIGCI MYTLLVGKPP

    FETSCLKETY LRIKKNEYSI PKHINPVAAS LIQKMLQTDP TARPTINELL

    NDEFFTSGYI PARLPITCLT IPPRFSIAPS SLDPSNRKPL TVLNKGLENP

    LPERPREKEE PVVRETGEVV DCHLSDMLQQ LHSVNASKPS ERGLVRQEEA

    EDPACIPIFW VSKWVDYSDK YGLGYQLCDN SVGVLFNDST RLILYNDGDS

    LQYIERDGTE SYLTVSSHPN SLMKKITLLK YFRNYMSEHL LKAGANITPR

    EGDELARLPY LRTWFRTRSA IILHLSNGSV QINFFQDHTK LILCPLMAAV

    TYIDEKRDFR TYRLSLLEEY GCCKELASRL RYARTMVDKL LSSRSASNRL

    KAS

  • UCSC uc002dlz.2; human.;



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