Human PGF ELISA Kit

Alternative name
Human PLGF ELISA Kit;Human PGFL ELISA Kit;Human D12S1900 ELISA Kit;Human PIGF ELISA Kit;Human PlGF-2 ELISA Kit;Human SHGC-10760 ELISA Kit;Human placental growth factor ELISA Kit;Human placenta growth factor ELISA Kit;Human placental growth factor, vascular endothelial growth factor-related protein ELISA Kit;
Catalog
E029906
species
Human
GenePGF
SpecificityNatural and recombinant human PLGF
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 1pg/ml
Intended UseHuman PGF ELISA Kit allows for the in vitro quantitative determination of PGF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human PLGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PLGF has been precoated onto 96-well plates. Standards (E.coli,A21-R149) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PLGF amount of sample captured in plate. Background: The protein, also called PGF, is 149 amino acids long and shares 53% identity with the platelet-derived growth factor-like region of human VPF. And the N-glycosylated PLGF protein is secreted into the medium and that it functions as a dimer1. The PLGF gene is mapped to 14q24-q31. There are 3 isoforms of PGF, designated PGF1,PGF2, and PGF3. Only PGF2 is able to bind heparin. Additionally, PGF regulates inter- and intramolecular cross-talk between the VEGF receptor tyrosine kinases FLT1 and FLK12. It also can stimulate angiogenesis andcollateral growth in ischemic heart and limb with at least a comparable efficiency to VEGF3.
Human Placenta growth factor Protein information
Uniprot ID PLGF_HUMAN
Uniprot AC P49763; Q07101; Q9BV78; Q9Y6S8;
UniGene Hs.252820;
GeneID 5228
KEGG hsa:5228;
Human Placenta growth factor Protein SEQUENCE
SEQUENCE 221 AA; 24789 MW; D364C6A73C1C6987 CRC64;
MPVMRLFPCF LQLLAGLALP AVPPQQWALS AGNGSSEVEV VPFQEVWGRS
YCRALERLVD VVSEYPSEVE HMFSPSCVSL LRCTGCCGDE NLHCVPVETA
NVTMQLLKIR SGDRPSYVEL TFSQHVRCEC RHSPGRQSPD MPGDFRADAP
SFLPPRRSLP MLFRMEWGCA LTGSQSAVWP SSPVPEEIPR MHPGRNGKKQ
QRKPLREKMK PERCGDAVPR R
UCSC uc001xrb.4; human. [P49763-1];

Human PGF ELISA Kit

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