Human PDGFA ELISA Kit

Alternative name
Human PDGF subunit A ELISA Kit;Human PDGF-1 ELISA Kit;Human Platelet-derived growth factor A chain ELISA Kit;Human platelet-derived growth factor alpha polypeptide ELISA Kit;Human PDGF1 ELISA Kit;Human PDGF-A ELISA Kit;Human platelet derived growth factor subunit A ELISA Kit;Human platelet-derived growth factor subunit A ELISA Kit;Human PDGF A-chain ELISA Kit;Human platelet-derived growth factor A-chain ELISA Kit;Human platelet-derived growth factor alpha chain ELISA Kit;
Catalog
E029685
species
Human
GenePDGFA
SpecificityThis assay has high sensitivity and excellent specificity for detection of PDGFA. No significant cross-reactivity or interference between PDGFA and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of PDGFA is typically less than 0.056ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding tw
Intended UseHuman PDGFA ELISA Kit allows for the in vitro quantitative determination of PDGFA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDGFA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PDGFA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDGFA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PDGFA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Platelet-derived growth factor subunit A Protein information
Uniprot ID PDGFA_HUMAN
Uniprot AC P04085; B5BU73;
UniGene Hs.535898;
GeneID 5154
KEGG hsa:5154;
Human Platelet-derived growth factor subunit A Protein SEQUENCE
SEQUENCE 211 AA; 24043 MW; 48633DDE558EFA43 CRC64;
MRTLACLLLL GCGYLAHVLA EEAEIPREVI ERLARSQIHS IRDLQRLLEI
DSVGSEDSLD TSLRAHGVHA TKHVPEKRPL PIRRKRSIEE AVPAVCKTRT
VIYEIPRSQV DPTSANFLIW PPCVEVKRCT GCCNTSSVKC QPSRVHHRSV
KVAKVEYVRK KPKLKEVQVR LEEHLECACA TTSLNPDYRE EDTGRPRESG
KKRKRKRLKP T
UCSC uc003sir.4; human. [P04085-1];

Human PDGFA ELISA Kit

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